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3vop

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Structure of Vaccinia virus A27

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 <StructureSection load='3vop' size='340' side='right'caption='[[3vop]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3vop]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccw Vaccw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VOP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VOP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3vop]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_WR Vaccinia virus WR]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VOP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VOP FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">A27, A27L, VACWR150 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10254 VACCW])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vop OCA], [https://pdbe.org/3vop PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vop RCSB], [https://www.ebi.ac.uk/pdbsum/3vop PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vop ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/A27_VACCW A27_VACCW]] Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion.<ref>PMID:3806791</ref> <ref>PMID:10640541</ref>
+[https://www.uniprot.org/uniprot/A27_VACCW A27_VACCW] Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion.<ref>PMID:3806791</ref> <ref>PMID:10640541</ref>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Vaccinia virus envelope protein A27 has multiple functions and is conserved in the Orthopoxvirus genus of the poxvirus family. A27 protein binds to cell surface heparan sulfate, provides an anchor for A26 protein packaging into mature virions, and is essential for egress of mature virus (MV) from infected cells. Here, we crystallized and determined the structure of a truncated form of A27 containing amino acids 21-84, C71/72A (tA27) at 2.2 A resolution. tA27 protein uses the N-terminal region interface (NTR) to form an unexpected trimeric assembly as the basic unit, which contains two parallel alpha-helices and one unusual antiparallel alpha-helix; in a serpentine way, two trimers stack with each other to form a hexamer using the C-terminal region interface (CTR). Recombinant tA27 protein forms oligomers in a concentration-dependent manner in vitro in gel filtration. Analytical ultracentrifugation and multi-angle light scattering revealed that tA27 dimerized in solution and that Leu47, Leu51, and Leu54 at the NTR and Ile68, Asn75, and Leu82 at the CTR are responsible for tA27 self-assembly in vitro. Finally, we constructed recombinant vaccinia viruses expressing full length mutant A27 protein defective in either NTR, CTR, or both interactions; the results demonstrated that wild type A27 dimer/trimer formation was impaired in NTR and CTR mutant viruses, resulting in small plaques that are defective in MV egress. Furthermore, the ability of A27 protein to form disulfide-linked protein complexes with A26 protein was partially or completely interrupted by NTR and CTR mutations, resulting in mature virion progeny with increased plasma membrane fusion activity upon cell entry. Together, these results demonstrate that A27 protein trimer structure is critical for MV egress and membrane fusion modulation. Because A27 is a neutralizing target, structural information will aid the development of inhibitors to block A27 self-assembly or complex formation against vaccinia virus infection.
-Crystal structure of vaccinia viral A27 protein reveals a novel structure critical for its function and complex formation with A26 protein.,Chang TH, Chang SJ, Hsieh FL, Ko TP, Lin CT, Ho MR, Wang I, Hsu ST, Guo RT, Chang W, Wang AH PLoS Pathog. 2013;9(8):e1003563. doi: 10.1371/journal.ppat.1003563. Epub 2013 Aug, 22. PMID:23990784<ref>PMID:23990784</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 3vop" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 24: / Line 15: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Vaccw]]
+[[Category: Vaccinia virus WR]]
-[[Category: Chang, T H]]
+[[Category: Chang TH]]
-[[Category: Hsieh, F L]]
+[[Category: Hsieh FL]]
-[[Category: Ko, T P]]
+[[Category: Ko TP]]
-[[Category: Wang, A H.J]]
+[[Category: Wang AHJ]]
-[[Category: Coiled-coil protein]]
-[[Category: Vaccinia virus]]
-[[Category: Viral protein]]
-[[Category: Virus envelope protein]]
-[[Category: Virus fusion protein]]

3vop

From Proteopedia

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Structure of Vaccinia virus A27

Structural highlights

Function

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