3vov

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Current revision (08:41, 20 March 2024) (edit) (undo)
 
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<StructureSection load='3vov' size='340' side='right'caption='[[3vov]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
<StructureSection load='3vov' size='340' side='right'caption='[[3vov]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3vov]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VOV FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3vov]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VOV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.02&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTHA0299 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Hexokinase Hexokinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.1 2.7.1.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vov OCA], [https://pdbe.org/3vov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vov RCSB], [https://www.ebi.ac.uk/pdbsum/3vov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vov ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vov OCA], [https://pdbe.org/3vov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vov RCSB], [https://www.ebi.ac.uk/pdbsum/3vov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vov ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q5SLJ4_THET8 Q5SLJ4_THET8]
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We characterized and determined the crystal structure of a putative glucokinase/hexokinase from Thermus thermophilus that belongs to the ROK (bacterial repressors, uncharacterized open reading frames, and sugar kinases) family. The protein possessed significant enzymatic activity against glucose and mannose, with V(max) values of 260 and 68mumol.min(-1).mg(-1) protein, respectively. Therefore, we concluded that the enzyme is a hexokinase. However, the hexokinase showed little catalytic capacity for galactose and fructose. Circular dichroism measurements indicated that the enzyme was structurally stable at 90 degrees C. The crystal structure of the enzyme was determined at a resolution of 2.02A, with R(cryst) and R(free) values of 18.1% and 22.6%, respectively. The polypeptide structure was divided into large and small domains. The ROK consensus sequences 1 and 2 were included in the large domain. The cysteine-rich consensus sequence 2 folded into a zinc finger, and the bound zinc was confirmed by both electron density and X-ray absorption fine structure (XAFS) spectrum. The overall structure was a homotetramer that consisted of a dimer of dimers. The accessible surface area buried by the association of the dimers into the tetrameric structures was significantly higher in the T. thermophilus enzyme than in a homologous tetrameric ROK sugar kinase.
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Characterization and crystal structure of the thermophilic ROK hexokinase from Thermus thermophilus.,Nakamura T, Kashima Y, Mine S, Oku T, Uegaki K J Biosci Bioeng. 2012 May 14. PMID:22591843<ref>PMID:22591843</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3vov" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Hexokinase 3D structures|Hexokinase 3D structures]]
*[[Hexokinase 3D structures|Hexokinase 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Hexokinase]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Thet8]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Kashima, Y]]
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[[Category: Kashima Y]]
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[[Category: Mine, S]]
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[[Category: Mine S]]
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[[Category: Nakamura, T]]
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[[Category: Nakamura T]]
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[[Category: Oku, T]]
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[[Category: Oku T]]
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[[Category: Uegaki, K]]
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[[Category: Uegaki K]]
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[[Category: Glucokinase]]
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[[Category: Rok]]
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[[Category: Sugar kinase]]
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[[Category: Transferase]]
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Current revision

Crystal Structure of ROK Hexokinase from Thermus thermophilus

PDB ID 3vov

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