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3wmu

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Current revision (08:46, 20 March 2024) (edit) (undo)
 
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<StructureSection load='3wmu' size='340' side='right'caption='[[3wmu]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
<StructureSection load='3wmu' size='340' side='right'caption='[[3wmu]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3wmu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WMU FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3wmu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mytilus_galloprovincialis Mytilus galloprovincialis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WMU FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wmv|3wmv]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wmu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wmu OCA], [https://pdbe.org/3wmu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wmu RCSB], [https://www.ebi.ac.uk/pdbsum/3wmu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wmu ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wmu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wmu OCA], [https://pdbe.org/3wmu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wmu RCSB], [https://www.ebi.ac.uk/pdbsum/3wmu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wmu ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/LEC1_MYTGA LEC1_MYTGA] Alpha-D-galactose-binding lectin (PubMed:27187419, PubMed:23093409, PubMed:27321048). Binds D-GalNAc, but not glucose or its derivatives (PubMed:27321048). Has hemagglutinating activity towards rabbit erythrocytes (PubMed:23093409, PubMed:27321048). Agglutinates bacteria. Has bacteriostatic activity on both Gram-positive and Gram-negative bacteria including B.subtilis, S.aureus, E.coli and V.parahaemolyticus, respectively (PubMed:27187419). Has a dose-dependent cytotoxic effect on the human globotriaosylceramide (Gb3)-expressing Epstein-Barr virus (EBV)-positive Burkitt's lymphoma (Raji) cell line (PubMed:23093409, PubMed:27321048). Has dose-dependent cytotoxic effect on another Burkitt's lymphoma (Ramos) cell line, which does not possess the EBV genome, but also expresses Gb3. Binds to Gb3 in these cells leading to phosphorylation of MEK1/2, ERK1/2, JNK and p38 kinase, activation of caspase-9/3 and to expression of p21 and tumor necrosis factor (TNF)-alpha (PubMed:26694420). No cytotoxic effect on the human chronic myelogenous leukemia (K-562) cell line, which does not express Gb3 (PubMed:23093409, PubMed:26694420). May be involved in innate immunity acting as an antibacterial or antifungal agent (PubMed:26694420, PubMed:27321048). May be a pattern recognition receptor (PRR) involved in recognition of glycans found on parasitic or symbiotic microorganisms (PubMed:27187419).<ref>PMID:23093409</ref> <ref>PMID:26694420</ref> <ref>PMID:27187419</ref> <ref>PMID:27321048</ref>
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MytiLec is a lectin, isolated from bivalves, with cytotoxic activity against cancer cell lines that express globotriaosyl ceramide, Galalpha(1,4)Galbeta(1,4)Glcalpha1-Cer, on the cell surface. Functional analysis shows that the protein binds to the disaccharide melibiose, Galalpha(1,6)Glc, and the trisaccharide globotriose, Galalpha(1,4)Galbeta(1,4)Glc. Recombinant MytiLec expressed in bacteria showed the same haemagglutinating and cytotoxic activity against Burkitt's lymphoma (Raji) cells as the native form. The crystal structure has been determined to atomic resolution, in the presence and absence of ligands, showing the protein to be a member of the beta-trefoil family, but with a mode of ligand binding unique to a small group of related trefoil lectins. Each of the three pseudo-equivalent binding sites within the monomer shows ligand binding, and the protein forms a tight dimer in solution. An engineered monomer mutant lost all cytotoxic activity against Raji cells, but retained some haemagglutination activity, showing that the quaternary structure of the protein is important for its cellular effects.
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Crystal structure of MytiLec, a galactose-binding lectin from the mussel Mytilus galloprovincialis with cytotoxicity against certain cancer cell types.,Terada D, Kawai F, Noguchi H, Unzai S, Hasan I, Fujii Y, Park SY, Ozeki Y, Tame JR Sci Rep. 2016 Jun 20;6:28344. doi: 10.1038/srep28344. PMID:27321048<ref>PMID:27321048</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3wmu" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Kawai, F]]
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[[Category: Mytilus galloprovincialis]]
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[[Category: Noguchi, H]]
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[[Category: Kawai F]]
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[[Category: Ozeki, Y]]
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[[Category: Noguchi H]]
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[[Category: Park, S Y]]
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[[Category: Ozeki Y]]
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[[Category: Tame, J R.H]]
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[[Category: Park S-Y]]
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[[Category: Terada, D]]
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[[Category: Tame JRH]]
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[[Category: Unzai, S]]
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[[Category: Terada D]]
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[[Category: Carbohydrate]]
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[[Category: Unzai S]]
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[[Category: Lectin]]
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[[Category: Sugar binding protein]]
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Current revision

The structure of an anti-cancer lectin mytilec apo-form from the mussel Mytilus galloprovincialis

PDB ID 3wmu

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