4u2h
From Proteopedia
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4u2h]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U2H FirstGlance]. <br> | <table><tr><td colspan='2'>[[4u2h]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U2H FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2h OCA], [https://pdbe.org/4u2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u2h RCSB], [https://www.ebi.ac.uk/pdbsum/4u2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2h ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2h OCA], [https://pdbe.org/4u2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u2h RCSB], [https://www.ebi.ac.uk/pdbsum/4u2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2h ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/Q8KNG3_MICEC Q8KNG3_MICEC] | [https://www.uniprot.org/uniprot/Q8KNG3_MICEC Q8KNG3_MICEC] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | CalE6 is a previously uncharacterized protein involved in the biosynthesis of calicheamicins in Micromonospora echinospora. It is a pyridoxal-5'-phosphate-dependent enzyme and exhibits high sequence homology to cystathionine gamma-lyases and cystathionine gamma-synthases. However, it was found to be active towards methionine and to convert this amino acid into alpha-ketobutyrate, ammonium, and methanethiol. The crystal structure of the cofactor-bound holoenzyme was resolved at 2.0 A; it contains two active site residues, Gly105 and Val322, specific for methionine gamma-lyases. Modeling of methionine into the active site allows identification of the active site residues responsible for substrate recognition and catalysis. These findings support that CalE6 is a putative methionine gamma-lyase producing methanethiol as a building block in biosynthesis of calicheamicins. | ||
| - | + | ==See Also== | |
| - | + | *[[Methionine gamma-lyase 3D structures|Methionine gamma-lyase 3D structures]] | |
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
The crystal structure of apo CalE6, a methionine gamma lyase from Micromonospora echinospora
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