5c22
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5c22]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C22 FirstGlance]. <br> | <table><tr><td colspan='2'>[[5c22]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C22 FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.302Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c22 OCA], [https://pdbe.org/5c22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c22 RCSB], [https://www.ebi.ac.uk/pdbsum/5c22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c22 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c22 OCA], [https://pdbe.org/5c22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c22 RCSB], [https://www.ebi.ac.uk/pdbsum/5c22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c22 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/HLYDC_ECOLX HLYDC_ECOLX] Involved in the transport of hemolysin A. | [https://www.uniprot.org/uniprot/HLYDC_ECOLX HLYDC_ECOLX] Involved in the transport of hemolysin A. | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The protein toxin HlyA of Escherichia coli is exported without a periplasmic intermediate by the type I secretion system (T1SS). The T1SS is composed of an inner membrane ABC transporter HlyB, an outer-membrane channel protein TolC, and a membrane fusion protein HlyD. However, the assembly of the T1SS remains to be elucidated. In this study, we determine the crystal structure of a part of the C-terminal periplasmic domain of HlyD. The long alpha-helical domain consisting of three alpha helices and a lipoyl domain was identified in the crystal structure. Based on the HlyD structure, we modeled the hexameric assembly of HlyD with a long alpha-helical barrel, which formed a complex with TolC in an intermeshing cogwheel-to-cogwheel manner, as observed in tripartite RND-type drug efflux pumps. These observations provide a structural blueprint for understanding the type I secretion system in pathogenic Gram-negative bacteria. | ||
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| - | Crystal Structure of a Soluble Fragment of the Membrane Fusion Protein HlyD in a Type I Secretion System of Gram-Negative Bacteria.,Kim JS, Song S, Lee M, Lee S, Lee K, Ha NC Structure. 2016 Jan 22. pii: S0969-2126(16)00005-8. doi:, 10.1016/j.str.2015.12.012. PMID:26833388<ref>PMID:26833388</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 5c22" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Hemolysin 3D structures|Hemolysin 3D structures]] | *[[Hemolysin 3D structures|Hemolysin 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Crystal structure of Zn-bound HlyD from E. coli
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