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| | ==Crystal structure of Enp1== | | ==Crystal structure of Enp1== |
| - | <StructureSection load='5wwo' size='340' side='right' caption='[[5wwo]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='5wwo' size='340' side='right'caption='[[5wwo]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5wwo]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WWO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5wwo]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WWO FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ENP1, MEG1, YBR247C, YBR1635 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), LTV1, YKL143W, YKL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wwo OCA], [http://pdbe.org/5wwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wwo RCSB], [http://www.ebi.ac.uk/pdbsum/5wwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wwo ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wwo OCA], [https://pdbe.org/5wwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wwo RCSB], [https://www.ebi.ac.uk/pdbsum/5wwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wwo ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ENP1_YEAST ENP1_YEAST]] Required for normal export of the pre-40S particles from the nucleus to the cytoplasm. Its subcellular location and association with pre-40S subunit shifts from mixed cytoplasm/nucleus to all nuclear in RPS19 disruptions, suggesting it acts after the ribosomal protein.<ref>PMID:12628929</ref> <ref>PMID:16159874</ref> [[http://www.uniprot.org/uniprot/LTV1_YEAST LTV1_YEAST]] Involved in protein transport. Non-ribosomal factor required for efficient nuclear export of the ribosomal 40S subunit. Component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome.<ref>PMID:15611164</ref> <ref>PMID:16888326</ref> | + | [https://www.uniprot.org/uniprot/ENP1_YEAST ENP1_YEAST] Required for normal export of the pre-40S particles from the nucleus to the cytoplasm. Its subcellular location and association with pre-40S subunit shifts from mixed cytoplasm/nucleus to all nuclear in RPS19 disruptions, suggesting it acts after the ribosomal protein.<ref>PMID:12628929</ref> <ref>PMID:16159874</ref> |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of Saccharomyces cerevisiae 90S determined from three cryo-electron microscopy single particle reconstructions at 4.5 to 8.7 angstrom resolution. The majority of the density maps were modeled and assigned to specific RNA and protein components. The nascent ribosome is assembled into isolated native-like substructures that are stabilized by abundant assembly factors. The 5' external transcribed spacer and U3 snoRNA nucleate a large subcomplex that scaffolds the nascent ribosome. U3 binds four sites of pre-rRNA, including a novel site on helix 27 but not the 3' side of the central pseudoknot, and crucially organizes the 90S structure. The 90S model provides significant insight into the principle of small subunit assembly and the function of assembly factors.
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| - | Molecular architecture of the 90S small subunit pre-ribosome.,Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K Elife. 2017 Feb 28;6. pii: e22086. doi: 10.7554/eLife.22086. PMID:28244370<ref>PMID:28244370</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5wwo" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Ye, K]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Zhang, W]] | + | [[Category: Ye K]] |
| - | [[Category: Component of 90s preribosome]] | + | [[Category: Zhang W]] |
| - | [[Category: Rna binding protein]]
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