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| | <SX load='6nav' size='340' side='right' viewer='molstar' caption='[[6nav]], [[Resolution|resolution]] 4.10Å' scene=''> | | <SX load='6nav' size='340' side='right' viewer='molstar' caption='[[6nav]], [[Resolution|resolution]] 4.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6nav]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_islandicus_lal14/1 Sulfolobus islandicus lal14/1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NAV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NAV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6nav]] is a 21 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_islandicus_LAL14/1 Sulfolobus islandicus LAL14/1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NAV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NAV FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nav OCA], [http://pdbe.org/6nav PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nav RCSB], [http://www.ebi.ac.uk/pdbsum/6nav PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nav ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.1Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nav OCA], [https://pdbe.org/6nav PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nav RCSB], [https://www.ebi.ac.uk/pdbsum/6nav PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nav ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/M9UD72_SULIS M9UD72_SULIS] |
| - | Pili on the surface of Sulfolobus islandicus are used for many functions, and serve as receptors for certain archaeal viruses. The cells grow optimally at pH 3 and ~80 degrees C, exposing these extracellular appendages to a very harsh environment. The pili, when removed from cells, resist digestion by trypsin or pepsin, and survive boiling in sodium dodecyl sulfate or 5 M guanidine hydrochloride. We used electron cryo-microscopy to determine the structure of these filaments at 4.1 A resolution. An atomic model was built by combining the electron density map with bioinformatics without previous knowledge of the pilin sequence-an approach that should prove useful for assemblies where all of the components are not known. The atomic structure of the pilus was unusual, with almost one-third of the residues being either threonine or serine, and with many hydrophobic surface residues. While the map showed extra density consistent with glycosylation for only three residues, mass measurements suggested extensive glycosylation. We propose that this extensive glycosylation renders these filaments soluble and provides the remarkable structural stability. We also show that the overall fold of the archaeal pilin is remarkably similar to that of archaeal flagellin, establishing common evolutionary origins.
| + | |
| - | | + | |
| - | An extensively glycosylated archaeal pilus survives extreme conditions.,Wang F, Cvirkaite-Krupovic V, Kreutzberger MAB, Su Z, de Oliveira GAP, Osinski T, Sherman N, DiMaio F, Wall JS, Prangishvili D, Krupovic M, Egelman EH Nat Microbiol. 2019 May 20. pii: 10.1038/s41564-019-0458-x. doi:, 10.1038/s41564-019-0458-x. PMID:31110358<ref>PMID:31110358</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6nav" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Sulfolobus islandicus lal14/1]] | + | [[Category: Sulfolobus islandicus LAL14/1]] |
| - | [[Category: Cvirkaite-Krupovic, V]] | + | [[Category: Cvirkaite-Krupovic V]] |
| - | [[Category: Egelman, E H]] | + | [[Category: Egelman EH]] |
| - | [[Category: Krupovic, M]] | + | [[Category: Krupovic M]] |
| - | [[Category: Prangishvili, D]] | + | [[Category: Prangishvili D]] |
| - | [[Category: Wang, F]] | + | [[Category: Wang F]] |
| - | [[Category: Archaeal pilus]]
| + | |
| - | [[Category: Helical symmetry]]
| + | |
| - | [[Category: Structural protein]]
| + | |
