1r8d
From Proteopedia
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'''Crystal Structure of MtaN Bound to DNA''' | '''Crystal Structure of MtaN Bound to DNA''' | ||
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[[Category: Brennan, R G.]] | [[Category: Brennan, R G.]] | ||
[[Category: Newberry, K J.]] | [[Category: Newberry, K J.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:12:45 2008'' | |
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Revision as of 04:12, 3 May 2008
Crystal Structure of MtaN Bound to DNA
Overview
Transcription regulators of the MerR family respond to myriad stress signals to activate sigma70/sigmaA-targeted genes, which contain suboptimal 19-bp spacers between their -35 and -10 promoter elements. The crystal structure of a BmrR-TPP(+)-DNA complex provided initial insight into the transcription activation mechanism of the MerR family, which involves base pair distortion, DNA undertwisting and shortening of the spacer, and realignment of the -35 and -10 boxes. Here, we describe the crystal structure of MerR family member MtaN bound to the mta promoter. Although the global DNA binding modes of MtaN and BmrR differ somewhat, homologous protein-DNA interactions are maintained. Moreover, despite their different sequences, the mta promoter conformation is essentially identical to that of the BmrR-TPP(+)-bound bmr promoter, indicating that this DNA distortion mechanism is common to the entire MerR family. Interestingly, DNA binding experiments reveal that the identity of the two central bases of the mta and bmr promoters, which are conserved as either a thymidine or an adenine in nearly all MerR promoters, is not important for DNA affinity. Comparison of the free and DNA-bound MtaN structures reveals that a conformational hinge, centered at residues N-terminal to the ubiquitous coiled coil, is key for mta promoter binding. Analysis of the structures of BmrR, CueR, and ZntR indicates that this hinge may be common to all MerR family members.
About this Structure
1R8D is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
The structural mechanism for transcription activation by MerR family member multidrug transporter activation, N terminus., Newberry KJ, Brennan RG, J Biol Chem. 2004 May 7;279(19):20356-62. Epub 2004 Feb 24. PMID:14985361 Page seeded by OCA on Sat May 3 07:12:45 2008
