1ema

<table><tr><td colspan='2'>[[1ema]] is a 1 chain structure. The June 2003 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Green Fluorescent Protein (GFP)'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2003_6 10.2210/rcsb_pdb/mom_2003_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1EMA FirstGlance]. <br>

</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ema FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ema OCA], [http://pdbe.org/1ema PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ema RCSB], [http://www.ebi.ac.uk/pdbsum/1ema PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ema ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

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== Publication Abstract from PubMed ==

The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria has generated intense interest as a marker for gene expression and localization of gene products. The chromophore, resulting from the spontaneous cyclization and oxidation of the sequence -Ser65 (or Thr65)-Tyr66-Gly67-, requires the native protein fold for both formation and fluorescence emission. The structure of Thr65 GFP has been determined at 1.9 angstrom resolution. The protein fold consists of an 11-stranded beta barrel with a coaxial helix, with the chromophore forming from the central helix. Directed mutagenesis of one residue adjacent to the chromophore, Thr203, to Tyr or His results in significantly red-shifted excitation and emission maxima.

Crystal structure of the Aequorea victoria green fluorescent protein.,Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075<ref>PMID:8703075</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 1ema" style="background-color:#fffaf0;"></div>

*[[GFP (Hebrew)|GFP (Hebrew)]]

*[[Gfp vc2|Gfp vc2]]

*[[Green Fluorescent Protein: Research Tool|Green Fluorescent Protein: Research Tool]]

*[[User:Joel L. Sussman/GFP Test Page|User:Joel L. Sussman/GFP Test Page]]

*[[User:Lynmarie K Thompson/2009Chem490|User:Lynmarie K Thompson/2009Chem490]]

*[[User:Shai Biran/ SAT|User:Shai Biran/ SAT]]

*[[User:Shai Biran/GFP test page|User:Shai Biran/GFP test page]]

*[[User:Shai Biran/Practice Proteopedia tutorial shai4|User:Shai Biran/Practice Proteopedia tutorial shai4]]

*[[User:Shai Biran/Practice Proteopedia tutorial shai5|User:Shai Biran/Practice Proteopedia tutorial shai5]]

*[[User:Shai Biran/Practice Proteopedia tutorial shai7|User:Shai Biran/Practice Proteopedia tutorial shai7]]

*[[User:Shai Biran/Practice Proteopedia tutorial shai9|User:Shai Biran/Practice Proteopedia tutorial shai9]]

== References ==

<references/>

[[Category: Ormo, M]]

[[Category: Remington, S J]]

[[Category: Fluorophore]]