1tpm
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(New page: 200px<br /> <applet load="1tpm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tpm" /> '''SOLUTION STRUCTURE OF THE FIBRIN BINDING FI...)
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Revision as of 17:20, 12 November 2007
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SOLUTION STRUCTURE OF THE FIBRIN BINDING FINGER DOMAIN OF TISSUE-TYPE PLASMINOGEN ACTIVATOR DETERMINED BY 1H NUCLEAR MAGNETIC RESONANCE
Contents |
Overview
The amino acid sequence of the first domain of tissue-type plasminogen, activator (t-PA) includes eight residues that are highly conserved in the, type 1 finger domains found in human fibronectin. A construct comprising, 50 residues from this finger domain of t-PA has been expressed and its, solution structure has been determined by two-dimensional nuclear magnetic, resonance spectroscopy. A total of 782 experimental restraints consisting, of 723 interproton distances derived from nuclear Overhauser effect, measurements, 43 torsion angles, and 16 hydrogen bond restraints were used, as the input for dynamical simulated annealing structure calculations., Twenty-eight structures were obtained that satisfied the experimental data, with no single distance violation greater than 0.3 A. The average atomic, root-mean-square distribution for the backbone atoms of the final, structures was 0.41 (+/- 0.13) A for the well defined part of the, structure (residues 4 to 47). The overall fold of the t-PA finger domain, shows a striking similarity to that of the seventh type 1 repeat of human, fibronectin with the side-chains of conserved residues lying in similar, conformations. One significant difference between the two molecules is, that hydrophobic residues cover the exposed surface of the principal, beta-sheet region in the t-PA finger domain. It is suggested that one face, of this region may interact with parts of the complete t-PA protein.
Disease
Known disease associated with this structure: Plasminogen activator deficiency OMIM:[173370]
About this Structure
1TPM is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of the fibrin binding finger domain of tissue-type plasminogen activator determined by 1H nuclear magnetic resonance., Downing AK, Driscoll PC, Harvey TS, Dudgeon TJ, Smith BO, Baron M, Campbell ID, J Mol Biol. 1992 Jun 5;225(3):821-33. PMID:1602484
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