1ra7

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[[Image:1ra7.jpg|left|200px]]
[[Image:1ra7.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1ra7 |SIZE=350|CAPTION= <scene name='initialview01'>1ra7</scene>, resolution 2.35&Aring;
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The line below this paragraph, containing "STRUCTURE_1ra7", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CAS:S-(DIMETHYLARSENIC)CYSTEINE'>CAS</scene>, <scene name='pdbligand=GTP:GUANOSINE-5&#39;-TRIPHOSPHATE'>GTP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= 3D ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12080 Human poliovirus 1])
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-->
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|DOMAIN=
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{{STRUCTURE_1ra7| PDB=1ra7 | SCENE= }}
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|RELATEDENTRY=[[1rdr|1RDR]], [[1ra6|1RA6]], [[1raj|1RAJ]], [[1tql|1TQL]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ra7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ra7 OCA], [http://www.ebi.ac.uk/pdbsum/1ra7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ra7 RCSB]</span>
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}}
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'''Poliovirus Polymerase with GTP'''
'''Poliovirus Polymerase with GTP'''
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[[Category: Thompson, A A.]]
[[Category: Thompson, A A.]]
[[Category: 3d]]
[[Category: 3d]]
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[[Category: gtp]]
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[[Category: Gtp]]
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[[Category: n-terminus]]
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[[Category: N-terminus]]
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[[Category: nucleotidyltransferase]]
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[[Category: Nucleotidyltransferase]]
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[[Category: poliovirus]]
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[[Category: Poliovirus]]
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[[Category: polymerase]]
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[[Category: Polymerase]]
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[[Category: rna-dependent]]
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[[Category: Rna-dependent]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:16:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:25:21 2008''
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Revision as of 04:16, 3 May 2008

Image:1ra7.jpg

Template:STRUCTURE 1ra7

Poliovirus Polymerase with GTP


Overview

The active RNA-dependent RNA polymerase of poliovirus, 3Dpol, is generated by cleavage of the 3CDpro precursor protein, a protease that has no polymerase activity despite containing the entire polymerase domain. By intentionally disrupting a known and persistent crystal packing interaction, we have crystallized the poliovirus polymerase in a new space group and solved the complete structure of the protein at 2.0 A resolution. It shows that the N-terminus of fully processed 3Dpol is buried in a surface pocket where it makes hydrogen bonds that act to position Asp238 in the active site. Asp238 is an essential residue that selects for the 2' OH group of substrate rNTPs, as shown by a 2.35 A structure of a 3Dpol-GTP complex. Mutational, biochemical, and structural data further demonstrate that 3Dpol activity is exquisitely sensitive to mutations at the N-terminus. This sensitivity is the result of allosteric effects where the structure around the buried N-terminus directly affects the positioning of Asp238 in the active site.

About this Structure

1RA7 is a Single protein structure of sequence from Human poliovirus 1. Full crystallographic information is available from OCA.

Reference

Structural basis for proteolysis-dependent activation of the poliovirus RNA-dependent RNA polymerase., Thompson AA, Peersen OB, EMBO J. 2004 Sep 1;23(17):3462-71. Epub 2004 Aug 12. PMID:15306852 Page seeded by OCA on Sat May 3 07:16:03 2008

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OCA

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