1rb4
From Proteopedia
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[[Image:1rb4.jpg|left|200px]] | [[Image:1rb4.jpg|left|200px]] | ||
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| - | + | {{STRUCTURE_1rb4| PDB=1rb4 | SCENE= }} | |
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'''ANTIPARALLEL TRIMER OF GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A TETRAGONAL AUTOMATIC SOLUTION''' | '''ANTIPARALLEL TRIMER OF GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A TETRAGONAL AUTOMATIC SOLUTION''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1RB4 is a [[Single protein]] structure | + | 1RB4 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RB4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Alber, T.]] | [[Category: Alber, T.]] | ||
[[Category: Holton, J.]] | [[Category: Holton, J.]] | ||
| - | [[Category: | + | [[Category: Automation]] |
| - | [[Category: | + | [[Category: Coiled coil]] |
| - | [[Category: | + | [[Category: Leucine zipper]] |
| - | [[Category: | + | [[Category: Peptide]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:17:36 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 04:17, 3 May 2008
ANTIPARALLEL TRIMER OF GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A TETRAGONAL AUTOMATIC SOLUTION
Overview
Efficient determination of protein crystal structures requires automated x-ray data analysis. Here, we describe the expert system ELVES and its use to determine automatically the structure of a 12-kDa protein. Multiwavelength anomalous diffraction analysis of a selenomethionyl derivative was used to image the Asn-16-Ala variant of the GCN4 leucine zipper. In contrast to the parallel, dimeric coiled coil formed by the WT sequence, the mutant unexpectedly formed an antiparallel trimer. This structural switch reveals how avoidance of core cavities at a single site can select the native fold of a protein. All structure calculations, including indexing, data processing, locating heavy atoms, phasing by multiwavelength anomalous diffraction, model building, and refinement, were completed without human intervention. The results demonstrate the feasibility of automated methods for determining high-resolution, x-ray crystal structures of proteins.
About this Structure
1RB4 is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Automated protein crystal structure determination using ELVES., Holton J, Alber T, Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1537-42. Epub 2004 Jan 29. PMID:14752198 Page seeded by OCA on Sat May 3 07:17:36 2008
