5x9a

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Current revision (10:17, 27 March 2024) (edit) (undo)
 
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<StructureSection load='5x9a' size='340' side='right'caption='[[5x9a]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
<StructureSection load='5x9a' size='340' side='right'caption='[[5x9a]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5x9a]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X9A OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5X9A FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5x9a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ciona_intestinalis Ciona intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X9A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X9A FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5x9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x9a OCA], [http://pdbe.org/5x9a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x9a RCSB], [http://www.ebi.ac.uk/pdbsum/5x9a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x9a ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x9a OCA], [https://pdbe.org/5x9a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x9a RCSB], [https://www.ebi.ac.uk/pdbsum/5x9a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x9a ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8T893_CIOIN Q8T893_CIOIN]
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Sperm chemotaxis toward a chemoattractant is very important for the success of fertilization. Calaxin, a member of the neuronal calcium sensor protein family, directly acts on outer-arm dynein and regulates specific flagellar movement during sperm chemotaxis of ascidian, Ciona intestinalis. Here, we present the crystal structures of calaxin both in the open and closed states upon Ca(2+) and Mg(2+) binding. The crystal structures revealed that three of the four EF-hands of a calaxin molecule bound Ca(2+) ions and that EF2 and EF3 played a critical role in the conformational transition between the open and closed states. The rotation of alpha7 and alpha8 helices induces a significant conformational change of a part of the alpha10 helix into the loop. The structural differences between the Ca(2+)- and Mg(2+)-bound forms indicates that EF3 in the closed state has a lower affinity for Mg(2+), suggesting that calaxin tends to adopt the open state in Mg(2+)-bound form. SAXS data supports that Ca(2+)-binding causes the structural transition toward the closed state. The changes in the structural transition of the C-terminal domain may be required to bind outer-arm dynein. These results provide a novel mechanism for recognizing a target protein using a calcium sensor protein.
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Crystal structure of a Ca(2+)-dependent regulator of flagellar motility reveals the open-closed structural transition.,Shojima T, Hou F, Takahashi Y, Matsumura Y, Okai M, Nakamura A, Mizuno K, Inaba K, Kojima M, Miyakawa T, Tanokura M Sci Rep. 2018 Jan 31;8(1):2014. doi: 10.1038/s41598-018-19898-7. PMID:29386625<ref>PMID:29386625</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5x9a" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Ciona intestinalis]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Hou, F]]
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[[Category: Hou F]]
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[[Category: Inaba, K]]
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[[Category: Inaba K]]
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[[Category: Miyakawa, T]]
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[[Category: Miyakawa T]]
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[[Category: Mizuno, K]]
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[[Category: Mizuno K]]
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[[Category: Okai, M]]
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[[Category: Okai M]]
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[[Category: Shojima, T]]
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[[Category: Shojima T]]
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[[Category: Takahashi, Y]]
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[[Category: Takahashi Y]]
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[[Category: Tanokura, M]]
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[[Category: Tanokura M]]
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[[Category: Calcium ion]]
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[[Category: Calcium-binding protein]]
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[[Category: Ef-hand]]
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[[Category: Metal binding protein]]
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[[Category: Neuronal calcium sensor]]
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Current revision

Crystal structure of calaxin with calcium

PDB ID 5x9a

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