5xlo

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Current revision (10:19, 27 March 2024) (edit) (undo)
 
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<SX load='5xlo' size='340' side='right' viewer='molstar' caption='[[5xlo]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
<SX load='5xlo' size='340' side='right' viewer='molstar' caption='[[5xlo]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5xlo]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XLO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XLO FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5xlo]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa], [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14] and [https://en.wikipedia.org/wiki/Pseudomonas_phage_JBD30 Pseudomonas phage JBD30]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XLO FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xlo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xlo OCA], [http://pdbe.org/5xlo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xlo RCSB], [http://www.ebi.ac.uk/pdbsum/5xlo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xlo ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xlo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xlo OCA], [https://pdbe.org/5xlo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xlo RCSB], [https://www.ebi.ac.uk/pdbsum/5xlo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xlo ProSAT]</span></td></tr>
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</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/CSY3_PSEAB CSY3_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Involved in crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref>
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[https://www.uniprot.org/uniprot/CSY3_PSEAB CSY3_PSEAB] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Involved in crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacteriophages encode anti-CRISPR suppressors to counteract the CRISPR/Cas immunity of their bacterial hosts, thus facilitating their survival and replication. Previous studies have shown that two phage-encoded anti-CRISPR proteins, AcrF1 and AcrF2, suppress the type I-F CRISPR/Cas system of Pseudomonas aeruginosa by preventing target DNA recognition by the Csy surveillance complex, but the precise underlying mechanism was unknown. Here we present the structure of AcrF1/2 bound to the Csy complex determined by cryo-EM single-particle reconstruction. By structural analysis, we found that AcrF1 inhibits target DNA recognition of the Csy complex by interfering with base pairing between the DNA target strand and crRNA spacer. In addition, multiple copies of AcrF1 bind to the Csy complex with different modes when working individually or cooperating with AcrF2, which might exclude target DNA binding through different mechanisms. Together with previous reports, we provide a comprehensive working scenario for the two anti-CRISPR suppressors, AcrF1 and AcrF2, which silence CRISPR/Cas immunity by targeting the Csy surveillance complex.
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Alternate binding modes of anti-CRISPR viral suppressors AcrF1/2 to Csy surveillance complex revealed by cryo-EM structures.,Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF Cell Res. 2017 Jul;27(7):853-864. doi: 10.1038/cr.2017.79. Epub 2017 Jun 2. PMID:28574055<ref>PMID:28574055</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5xlo" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Gao, G F]]
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[[Category: Pseudomonas aeruginosa]]
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[[Category: Peng, R]]
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[[Category: Pseudomonas aeruginosa UCBPP-PA14]]
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[[Category: Shi, Y]]
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[[Category: Pseudomonas phage JBD30]]
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[[Category: Anti-crispr protein]]
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[[Category: Gao GF]]
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[[Category: Csy complex]]
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[[Category: Peng R]]
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[[Category: Immune system-rna complex]]
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[[Category: Shi Y]]
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[[Category: Type i-f crispr/cas system]]
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Current revision

Anti-CRISPR proteins AcrF1/2 bound to Csy surveillance complex with a 32nt spacer crRNA backbone region

5xlo, resolution 3.80Å

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OCA

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