5xrl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:20, 27 March 2024) (edit) (undo)
 
Line 1: Line 1:
==Galectin-10/Charcot-Leyden crystal protein variant N65A crystal structure==
==Galectin-10/Charcot-Leyden crystal protein variant N65A crystal structure==
-
<StructureSection load='5xrl' size='340' side='right' caption='[[5xrl]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+
<StructureSection load='5xrl' size='340' side='right'caption='[[5xrl]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5xrl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XRL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XRL FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5xrl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XRL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XRL FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.004&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CLC, LGALS10, LGALS10A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xrl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xrl OCA], [http://pdbe.org/5xrl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xrl RCSB], [http://www.ebi.ac.uk/pdbsum/5xrl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xrl ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xrl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xrl OCA], [https://pdbe.org/5xrl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xrl RCSB], [https://www.ebi.ac.uk/pdbsum/5xrl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xrl ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/LEG10_HUMAN LEG10_HUMAN]] Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).<ref>PMID:17502455</ref>
+
[https://www.uniprot.org/uniprot/LEG10_HUMAN LEG10_HUMAN] Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).<ref>PMID:17502455</ref>
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Galectin-10 (Gal-10) which forms Charcot-Leyden crystals in vivo, is crucial to regulating lymph cell function. Here, we solved the crystal structures of Gal-10 and eight variants at resolutions of 1.55-2.00 A. Structural analysis and size exclusion chromatography demonstrated that Gal-10 dimerizes with a novel global shape that is different from that of other prototype galectins (e.g., Gal-1, -2 and -7). In the Gal-10 dimer, Glu33 from one subunit modifies the carbohydrate binding site of another, essentially inhibiting disaccharide binding. Nevertheless, glycerol (and possibly other small hydroxylated molecules) can interact with residues at the ligand binding site, with His53 being the most crucial for binding. Alanine substitution of the conserved Trp residue (Trp72) that is crucial to saccharide binding in other galectins, actually leads to enhanced erythrocyte agglutination, suggesting that Trp72 negatively regulates Gal-10 ligand binding. Overall, our crystallographic and biochemical results provide insight into Gal-10 ligand binding specificity.
+
-
Galectin-10: a new structural type of prototype galectin dimer and effects on saccharide ligand binding.,Su J, Gao J, Si Y, Cui L, Song C, Wang Y, Wu R, Tai G, Zhou Y Glycobiology. 2017 Dec 23. pii: 4773962. doi: 10.1093/glycob/cwx107. PMID:29293962<ref>PMID:29293962</ref>
+
==See Also==
-
 
+
*[[Galectin 3D structures|Galectin 3D structures]]
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5xrl" style="background-color:#fffaf0;"></div>
+
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Human]]
+
[[Category: Homo sapiens]]
-
[[Category: Su, J]]
+
[[Category: Large Structures]]
-
[[Category: Galectin-10/charcot-leyden crystal protein]]
+
[[Category: Su J]]
-
[[Category: Protein binding]]
+

Current revision

Galectin-10/Charcot-Leyden crystal protein variant N65A crystal structure

PDB ID 5xrl

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5xrl"
Personal tools