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| | ==Crystal structure of drosophila simulans Rhino chromoshadow domain in complex with N-terminal domain== | | ==Crystal structure of drosophila simulans Rhino chromoshadow domain in complex with N-terminal domain== |
| - | <StructureSection load='5xyw' size='340' side='right' caption='[[5xyw]], [[Resolution|resolution]] 2.71Å' scene=''> | + | <StructureSection load='5xyw' size='340' side='right'caption='[[5xyw]], [[Resolution|resolution]] 2.71Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5xyw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosi Drosi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XYW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5xyw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_simulans Drosophila simulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XYW FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rhi, Dsim\GD27086, Dsimw501_GD27086, GD25457 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7240 DROSI]), Dsim\GD21652, Dsim_GD21652, GD21652 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7240 DROSI])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.705Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyw OCA], [http://pdbe.org/5xyw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xyw RCSB], [http://www.ebi.ac.uk/pdbsum/5xyw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyw ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyw OCA], [https://pdbe.org/5xyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xyw RCSB], [https://www.ebi.ac.uk/pdbsum/5xyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyw ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q49BI5_DROSI Q49BI5_DROSI] |
| - | PIWI-interacting RNAs (piRNAs) silence transposons in germ cells to maintain genome stability and animal fertility. Rhino, a rapidly evolving heterochromatin protein 1 (HP1) family protein, binds Deadlock in a species-specific manner and so defines the piRNA-producing loci in the Drosophila genome. Here, we determine the crystal structures of Rhino-Deadlock complex in Drosophila melanogaster and simulans In both species, one Rhino binds the N-terminal helix-hairpin-helix motif of one Deadlock protein through a novel interface formed by the beta-sheet in the Rhino chromoshadow domain. Disrupting the interface leads to infertility and transposon hyperactivation in flies. Our structural and functional experiments indicate that electrostatic repulsion at the interaction interface causes cross-species incompatibility between the sibling species. By determining the molecular architecture of this piRNA-producing machinery, we discover a novel HP1-partner interacting mode that is crucial to piRNA biogenesis and transposon silencing. We thus explain the cross-species incompatibility of two sibling species at the molecular level.
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| - | Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification.,Yu B, Lin YA, Parhad SS, Jin Z, Ma J, Theurkauf WE, Zhang ZZ, Huang Y EMBO Rep. 2018 Jun 1. pii: embr.201745418. doi: 10.15252/embr.201745418. PMID:29858487<ref>PMID:29858487</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5xyw" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drosi]] | + | [[Category: Drosophila simulans]] |
| - | [[Category: Huang, Y]] | + | [[Category: Large Structures]] |
| - | [[Category: Yu, B W]] | + | [[Category: Huang Y]] |
| - | [[Category: Chromoshadow]] | + | [[Category: Yu BW]] |
| - | [[Category: Complex]]
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| - | [[Category: Protein binding]]
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