1rbn

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[[Image:1rbn.gif|left|200px]]
[[Image:1rbn.gif|left|200px]]
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{{Structure
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|PDB= 1rbn |SIZE=350|CAPTION= <scene name='initialview01'>1rbn</scene>, resolution 2.1&Aring;
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The line below this paragraph, containing "STRUCTURE_1rbn", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=P5P:PURINE+RIBOSIDE-5&#39;-MONOPHOSPHATE'>P5P</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span>
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|GENE=
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{{STRUCTURE_1rbn| PDB=1rbn | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rbn OCA], [http://www.ebi.ac.uk/pdbsum/1rbn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rbn RCSB]</span>
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'''THE STRUCTURE OF RIBONUCLEASE A DERIVATIVE II AT 2.1 ANGSTROMS RESOLUTION'''
'''THE STRUCTURE OF RIBONUCLEASE A DERIVATIVE II AT 2.1 ANGSTROMS RESOLUTION'''
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[[Category: Boque, L.]]
[[Category: Boque, L.]]
[[Category: Fita, I.]]
[[Category: Fita, I.]]
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[[Category: hydrolase(nucleic acid,rna)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:18:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:25:53 2008''
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Revision as of 04:18, 3 May 2008

Image:1rbn.gif

Template:STRUCTURE 1rbn

THE STRUCTURE OF RIBONUCLEASE A DERIVATIVE II AT 2.1 ANGSTROMS RESOLUTION


Overview

The crystal structure of bovine pancreatic ribonuclease A derivative II, a covalent derivative obtained by reaction of 6-chloropurine 9-beta-D-ribofuranosyl 5'-monophosphate with the alpha-amino group of Lys-1, has been determined and refined at 2.1-A resolution with an agreement factor R = 0.166 for 6254 reflections in the resolution shell 8.0 to 2.1 A. Crystals are orthorhombic and belong to space group C222(1) with unit cell parameters a = 75.73 A, b = 57.85 A, and c = 53.26 A. This crystal packing had never been reported before for pancreatic ribonuclease nor its complexes. The structure found is in accordance with the location of p2, B3, and R3 subsites at the N-terminal region of the protein and provides an explanation of the catalytic behavior observed for this derivative. In particular, differences in kinetic parameters and in the pKa value of His-119 between derivative II and native ribonuclease A can be interpreted on the basis of the position of the phosphate moiety within the derivative structure. Some uncertainty remains on the nucleotide sugar conformation determined.

About this Structure

1RBN is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Structure of ribonuclease A derivative II at 2.1-A resolution., Boque L, Gracia Coll M, Vilanova M, Cuchillo CM, Fita I, J Biol Chem. 1994 Aug 5;269(31):19707-12. PMID:8051049 Page seeded by OCA on Sat May 3 07:18:26 2008

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