5yd0

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==Crystal structure of Schlafen 13 (SLFN13) N'-domain==
==Crystal structure of Schlafen 13 (SLFN13) N'-domain==
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<StructureSection load='5yd0' size='340' side='right' caption='[[5yd0]], [[Resolution|resolution]] 3.18&Aring;' scene=''>
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<StructureSection load='5yd0' size='340' side='right'caption='[[5yd0]], [[Resolution|resolution]] 3.18&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5yd0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YD0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YD0 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5yd0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YD0 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.182&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Slfn13, Slfn8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yd0 OCA], [http://pdbe.org/5yd0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yd0 RCSB], [http://www.ebi.ac.uk/pdbsum/5yd0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yd0 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yd0 OCA], [https://pdbe.org/5yd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yd0 RCSB], [https://www.ebi.ac.uk/pdbsum/5yd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yd0 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/SLN13_RAT SLN13_RAT] Endoribonuclease that cleaves tRNAs and rRNAs (PubMed:29563550). Cleaves tRNAs 11 nucleotides from the 3'-terminus at the acceptor stem (PubMed:29563550). Does not act on tRNA(Sec) (PubMed:29563550).<ref>PMID:29563550</ref>
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Cleavage of transfer (t)RNA and ribosomal (r)RNA are critical and conserved steps of translational control for cells to overcome varied environmental stresses. However, enzymes that are responsible for this event have not been fully identified in high eukaryotes. Here, we report a mammalian tRNA/rRNA-targeting endoribonuclease: SLFN13, a member of the Schlafen family. Structural study reveals a unique pseudo-dimeric U-pillow-shaped architecture of the SLFN13 N'-domain that may clamp base-paired RNAs. SLFN13 is able to digest tRNAs and rRNAs in vitro, and the endonucleolytic cleavage dissevers 11 nucleotides from the 3'-terminus of tRNA at the acceptor stem. The cytoplasmically localised SLFN13 inhibits protein synthesis in 293T cells. Moreover, SLFN13 restricts HIV replication in a nucleolytic activity-dependent manner. According to these observations, we term SLFN13 RNase S13. Our study provides insights into the modulation of translational machinery in high eukaryotes, and sheds light on the functional mechanisms of the Schlafen family.
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Structure of Schlafen13 reveals a new class of tRNA/rRNA- targeting RNase engaged in translational control.,Yang JY, Deng XY, Li YS, Ma XC, Feng JX, Yu B, Chen Y, Luo YL, Wang X, Chen ML, Fang ZX, Zheng FX, Li YP, Zhong Q, Kang TB, Song LB, Xu RH, Zeng MS, Chen W, Zhang H, Xie W, Gao S Nat Commun. 2018 Mar 21;9(1):1165. doi: 10.1038/s41467-018-03544-x. PMID:29563550<ref>PMID:29563550</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5yd0" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Buffalo rat]]
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[[Category: Large Structures]]
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[[Category: Gao, S]]
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[[Category: Rattus norvegicus]]
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[[Category: Yang, J Y]]
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[[Category: Gao S]]
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[[Category: Anti-hiv]]
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[[Category: Yang J-Y]]
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[[Category: Endonuclease]]
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[[Category: Hydrolase]]
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[[Category: Rrna]]
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[[Category: Trna]]
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Current revision

Crystal structure of Schlafen 13 (SLFN13) N'-domain

PDB ID 5yd0

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