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| | ==Crystal structure of Utp30== | | ==Crystal structure of Utp30== |
| - | <StructureSection load='5ydu' size='340' side='right' caption='[[5ydu]], [[Resolution|resolution]] 2.65Å' scene=''> | + | <StructureSection load='5ydu' size='340' side='right'caption='[[5ydu]], [[Resolution|resolution]] 2.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ydu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YDU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YDU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ydu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YDU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YDU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.646Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UTP30, YKR060W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ydu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ydu OCA], [http://pdbe.org/5ydu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ydu RCSB], [http://www.ebi.ac.uk/pdbsum/5ydu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ydu ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ydu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ydu OCA], [https://pdbe.org/5ydu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ydu RCSB], [https://www.ebi.ac.uk/pdbsum/5ydu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ydu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RL1D1_YEAST RL1D1_YEAST]] Involved in rRNA-processing and ribosome biosynthesis.<ref>PMID:16544271</ref> | + | [https://www.uniprot.org/uniprot/RL1D1_YEAST RL1D1_YEAST] Involved in rRNA-processing and ribosome biosynthesis.<ref>PMID:16544271</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The 90S pre-ribosomes are gigantic early assembly intermediates of small ribosomal subunits. Cryo-EM structures of 90S were recently determined, but many of its components have not been accurately modelled. Here we determine the crystal structure of yeast Utp30, a ribosomal L1 domain-containing protein in 90S, at 2.65 A resolution, revealing a classic two-domain fold. The structure of Utp30 fits well into the cryo-EM density of 90S, confirming its previously assigned location. Utp30 binds to the rearranged helix 41 of 18S rRNA and helix 4 of 5' external transcribed spacer in 90S. Comparison of RNA-binding modes of different L1 domains illustrates that they consistently recognize a short RNA duplex with the concaved surface of domain I, but are versatile in RNA recognition outside the core interface. Cic1 is a paralog of Utp30 associating with large subunit pre-ribosomes. Utp30 and Cic1 share similar RNA binding mode, suggesting that their distinct functions may be executed by a single protein in other organisms. Deletion of Utp30 does not affect the composition of 90S. The nonessential role of Utp30 could be ascribed to its peripheral localization and redundant interactions in 90S.
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| - | Structure and RNA recognition of ribosome assembly factor Utp30.,Hu J, Zhu X, Ye K RNA. 2017 Sep 26. pii: rna.062695.117. doi: 10.1261/rna.062695.117. PMID:28951391<ref>PMID:28951391</ref>
| + | ==See Also== |
| - | | + | *[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5ydu" style="background-color:#fffaf0;"></div>
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Hu, J]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Ye, K]] | + | [[Category: Hu J]] |
| - | [[Category: Zhu, X]] | + | [[Category: Ye K]] |
| - | [[Category: 90s pre-ribosome]] | + | [[Category: Zhu X]] |
| - | [[Category: Ribosomal protein]]
| + | |
| - | [[Category: Ribosome assembly]]
| + | |
