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| | <StructureSection load='5yet' size='340' side='right'caption='[[5yet]], [[Resolution|resolution]] 2.81Å' scene=''> | | <StructureSection load='5yet' size='340' side='right'caption='[[5yet]], [[Resolution|resolution]] 2.81Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5yet]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Apmv Apmv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YET OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YET FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5yet]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acanthamoeba_polyphaga_mimivirus Acanthamoeba polyphaga mimivirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YET FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MIMI_R354 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=212035 APMV])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.806Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yet OCA], [http://pdbe.org/5yet PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yet RCSB], [http://www.ebi.ac.uk/pdbsum/5yet PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yet ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yet OCA], [https://pdbe.org/5yet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yet RCSB], [https://www.ebi.ac.uk/pdbsum/5yet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yet ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/YR354_MIMIV YR354_MIMIV] |
| - | A clustered regularly interspaced short palindromic repeats (CRISPR)-like "mimivirus virophage resistance element" (MIMIVIRE) system, which contains specific cascade genes and a CRISPR array against virophages, was reported in mimiviruses. An essential component of the MIMIVIRE system is R354, encoding a nuclease and a likely functional homolog of Cas4. Here we show that R354 is a dual nuclease with both exonuclease and endonuclease activities. Structural analysis revealed that the catalytic core domain of R354 is similar to those of Cas4 and ? exonuclease despite their low sequence identity. R354 forms a homodimer that is important for its exonuclease but not endonuclease activity. Structural comparisons between the active and semi-active states of R354 demonstrated that an activation loop adjacent to the catalytic site is critical for enzymatic activity. Overall, the results suggest that R354 belongs to a novel MIMIVIRE system involved in innate virus immunity and provides a template for the identification of new CRISPR systems in other species.
| + | |
| - | | + | |
| - | Structural and Mechanistic Analyses Reveal a Unique Cas4-like Protein in the Mimivirus Virophage Resistance Element System.,Dou C, Yu M, Gu Y, Wang J, Yin K, Nie C, Zhu X, Qi S, Wei Y, Cheng W iScience. 2018 May 25;3:1-10. doi: 10.1016/j.isci.2018.04.001. Epub 2018 Apr 11. PMID:30428313<ref>PMID:30428313</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5yet" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Apmv]] | + | [[Category: Acanthamoeba polyphaga mimivirus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cheng, W]] | + | [[Category: Cheng W]] |
| - | [[Category: Dou, C]] | + | [[Category: Dou C]] |
| - | [[Category: Gu, Y J]] | + | [[Category: Gu YJ]] |
| - | [[Category: Yu, M J]] | + | [[Category: Yu MJ]] |
| - | [[Category: Cas4-like]]
| + | |
| - | [[Category: Mimivire]]
| + | |
| - | [[Category: Nuclear protein]]
| + | |
| - | [[Category: Nuclease]]
| + | |
| - | [[Category: R354]]
| + | |
