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| <SX load='5yfp' size='340' side='right' viewer='molstar' caption='[[5yfp]], [[Resolution|resolution]] 4.40Å' scene=''> | | <SX load='5yfp' size='340' side='right' viewer='molstar' caption='[[5yfp]], [[Resolution|resolution]] 4.40Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[5yfp]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YFP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5YFP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5yfp]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YFP FirstGlance]. <br> |
- | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5yfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yfp OCA], [http://pdbe.org/5yfp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yfp RCSB], [http://www.ebi.ac.uk/pdbsum/5yfp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yfp ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.4Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yfp OCA], [https://pdbe.org/5yfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yfp RCSB], [https://www.ebi.ac.uk/pdbsum/5yfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yfp ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
- | [[http://www.uniprot.org/uniprot/SEC15_YEAST SEC15_YEAST]] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.<ref>PMID:1512289</ref> [[http://www.uniprot.org/uniprot/SEC6_YEAST SEC6_YEAST]] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. [[http://www.uniprot.org/uniprot/EXO70_YEAST EXO70_YEAST]] Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in the assembly of the exocyst.<ref>PMID:15583030</ref> [[http://www.uniprot.org/uniprot/SEC5_YEAST SEC5_YEAST]] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. [[http://www.uniprot.org/uniprot/EXO84_YEAST EXO84_YEAST]] Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in both the assembly of the exocyst and the polarization of this complex to specific sites of the plasma membrane for exocytosis and to the budding site. Also involved in assembly of the spliceosome.<ref>PMID:10438536</ref> <ref>PMID:11425851</ref> <ref>PMID:15788396</ref> [[http://www.uniprot.org/uniprot/SEC3_YEAST SEC3_YEAST]] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. [[http://www.uniprot.org/uniprot/SEC8_YEAST SEC8_YEAST]] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. [[http://www.uniprot.org/uniprot/SEC10_YEAST SEC10_YEAST]] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. | + | [https://www.uniprot.org/uniprot/SEC3_YEAST SEC3_YEAST] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. |
- | <div style="background-color:#fffaf0;">
| + | |
- | == Publication Abstract from PubMed ==
| + | |
- | The exocyst is an evolutionarily conserved octameric protein complex that mediates the tethering of post-Golgi secretory vesicles to the plasma membrane during exocytosis and is implicated in many cellular processes such as cell polarization, cytokinesis, ciliogenesis and tumor invasion. Using cryo-EM and chemical cross-linking MS (CXMS), we solved the structure of the Saccharomyces cerevisiae exocyst complex at an average resolution of 4.4 A. Our model revealed the architecture of the exocyst and led to the identification of the helical bundles that mediate the assembly of the complex at its core. Sequence analysis suggests that these regions are evolutionarily conserved across eukaryotic systems. Additional cell biological data suggest a mechanism for exocyst assembly that leads to vesicle tethering at the plasma membrane.
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- | Cryo-EM structure of the exocyst complex.,Mei K, Li Y, Wang S, Shao G, Wang J, Ding Y, Luo G, Yue P, Liu JJ, Wang X, Dong MQ, Wang HW, Guo W Nat Struct Mol Biol. 2018 Jan 15. pii: 10.1038/s41594-017-0016-2. doi:, 10.1038/s41594-017-0016-2. PMID:29335562<ref>PMID:29335562</ref>
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- | | + | |
- | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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- | </div>
| + | |
- | <div class="pdbe-citations 5yfp" style="background-color:#fffaf0;"></div>
| + | |
- | == References ==
| + | |
- | <references/>
| + | |
| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
- | [[Category: Baker's yeast]] | |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
- | [[Category: Ding, Y]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
- | [[Category: Dong, M Q]] | + | [[Category: Ding Y]] |
- | [[Category: Guo, W]] | + | [[Category: Dong MQ]] |
- | [[Category: Li, Y]] | + | [[Category: Guo W]] |
- | [[Category: Liu, J J]] | + | [[Category: Li Y]] |
- | [[Category: Luo, G]] | + | [[Category: Liu JJ]] |
- | [[Category: Mei, K]] | + | [[Category: Luo G]] |
- | [[Category: Shao, G]] | + | [[Category: Mei K]] |
- | [[Category: Wang, H W]] | + | [[Category: Shao G]] |
- | [[Category: Wang, J]] | + | [[Category: Wang HW]] |
- | [[Category: Wang, S]] | + | [[Category: Wang J]] |
- | [[Category: Wang, X]] | + | [[Category: Wang S]] |
- | [[Category: Yue, P]] | + | [[Category: Wang X]] |
- | [[Category: Coiled-coil]]
| + | [[Category: Yue P]] |
- | [[Category: Exocyst]]
| + | |
- | [[Category: Exocytosis]]
| + | |