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| | <StructureSection load='5yo8' size='340' side='right'caption='[[5yo8]], [[Resolution|resolution]] 1.64Å' scene=''> | | <StructureSection load='5yo8' size='340' side='right'caption='[[5yo8]], [[Resolution|resolution]] 1.64Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5yo8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_alcalophilus_atcc_27647 Bacillus alcalophilus atcc 27647]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YO8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YO8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5yo8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Alkalihalobacillus_alcalophilus_ATCC_27647_=_CGMCC_1.3604 Alkalihalobacillus alcalophilus ATCC 27647 = CGMCC 1.3604]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YO8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YO8 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BALCAV_0202405 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1218173 Bacillus alcalophilus ATCC 27647])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yo8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yo8 OCA], [http://pdbe.org/5yo8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yo8 RCSB], [http://www.ebi.ac.uk/pdbsum/5yo8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yo8 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yo8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yo8 OCA], [https://pdbe.org/5yo8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yo8 RCSB], [https://www.ebi.ac.uk/pdbsum/5yo8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yo8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A094YZ24_ALKAL A0A094YZ24_ALKAL] |
| - | Thousands of terpenes have been identified to date. However, only two classes of enzymes are known to be involved in their biosynthesis, and each class has characteristic amino-acid motifs. We recently identified a novel large-terpene (C25/C30/C35) synthase, which shares no motifs with known enzymes. To elucidate the molecular mechanism of this enzyme, we determined the crystal structure of a large-beta-prene synthase from B. alcalophilus (BalTS). Surprisingly, the overall structure of BalTS is similar to that of the alpha-domain of class I terpene synthases although their primary structures are totally different from each other. Two novel aspartate-rich motifs, DYLDNLxD and DY(F,L,W)IDxxED, are identified, and mutations of any one of the aspartates eliminate its enzymatic activity. The present work leads us to propose a new subclass of terpene synthases, class IB, which is probably responsible for large-terpene biosynthesis.
| + | |
| - | | + | |
| - | Crystal structure and functional analysis of large-terpene synthases belonging to a newly found subclass.,Fujihashi M, Sato T, Tanaka Y, Yamamoto D, Nishi T, Ueda D, Murakami M, Yasuno Y, Sekihara A, Fuku K, Shinada T, Miki K Chem Sci. 2018 Mar 16;9(15):3754-3758. doi: 10.1039/c8sc00289d. eCollection 2018 , Apr 21. PMID:29780507<ref>PMID:29780507</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5yo8" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus alcalophilus atcc 27647]] | + | [[Category: Alkalihalobacillus alcalophilus ATCC 27647 = CGMCC 1 3604]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Fujihashi, M]] | + | [[Category: Fujihashi M]] |
| - | [[Category: Miki, K]] | + | [[Category: Miki K]] |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Terpene synthase]]
| + | |
