1rbw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1rbw.gif|left|200px]]
[[Image:1rbw.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1rbw |SIZE=350|CAPTION= <scene name='initialview01'>1rbw</scene>, resolution 1.69&Aring;
+
The line below this paragraph, containing "STRUCTURE_1rbw", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=GAI:GUANIDINE'>GAI</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1rbw| PDB=1rbw | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rbw OCA], [http://www.ebi.ac.uk/pdbsum/1rbw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rbw RCSB]</span>
+
-
}}
+
'''RIBONUCLEASE A (E.C.3.1.27.5) WITH GUANIDINIUM'''
'''RIBONUCLEASE A (E.C.3.1.27.5) WITH GUANIDINIUM'''
Line 28: Line 25:
[[Category: Dunbar, J L.H.]]
[[Category: Dunbar, J L.H.]]
[[Category: Farber, G K.]]
[[Category: Farber, G K.]]
-
[[Category: hydrolase (nucleic acid]]
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:18:54 2008''
-
[[Category: rna)]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:25:57 2008''
+

Revision as of 04:18, 3 May 2008

Image:1rbw.gif

Template:STRUCTURE 1rbw

RIBONUCLEASE A (E.C.3.1.27.5) WITH GUANIDINIUM


Overview

Virtually all studies of the protein-folding reaction add either heat, acid, or a chemical denaturant to an aqueous protein solution in order to perturb the protein structure. When chemical denaturants are used, very high concentrations are usually necessary to observe any change in protein structure. In a solution with such high denaturant concentrations, both the structure of the protein and the structure of the solvent around the protein can be altered. X-ray crystallography is the obvious experimental technique to probe both types of changes. In this paper, we report the crystal structures of dihydrofolate reductase with urea and of ribonuclease A with guanidinium chloride. These two classic denaturants have similar effects on the native structure of the protein. The most important change that occurs is a reduction in the overall thermal factor. These structures offer a molecular explanation for the reduction in mobility. Although the reduction is observed only with the native enzyme in the crystal, a similar decrease in mobility has also been observed in the unfolded state in solution (Makhatadze G, Privalov PL. 1992. Protein interactions with urea and guanidinium chloride: A calorimetric study.

About this Structure

1RBW is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

The effect of denaturants on protein structure., Dunbar J, Yennawar HP, Banerjee S, Luo J, Farber GK, Protein Sci. 1997 Aug;6(8):1727-33. PMID:9260285 Page seeded by OCA on Sat May 3 07:18:54 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rbw"
Personal tools