This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5z1g

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:26, 27 March 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='5z1g' size='340' side='right'caption='[[5z1g]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
<StructureSection load='5z1g' size='340' side='right'caption='[[5z1g]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5z1g]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z1G OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z1G FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5z1g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z1G FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.294&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EBP2, YKL172W, YKL636 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), BRX1, YOL077C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z1g OCA], [http://pdbe.org/5z1g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z1g RCSB], [http://www.ebi.ac.uk/pdbsum/5z1g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z1g ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z1g OCA], [https://pdbe.org/5z1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z1g RCSB], [https://www.ebi.ac.uk/pdbsum/5z1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z1g ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/EBP2_YEAST EBP2_YEAST]] Required for the processing of the 27S pre-rRNA. Probably involved in the step of the processing of the 27 SA precursor into the 27 SB intermediate.<ref>PMID:10849420</ref> <ref>PMID:10947841</ref> [[http://www.uniprot.org/uniprot/BRX1_YEAST BRX1_YEAST]] Required for biogenesis of the 60S ribosomal subunit.<ref>PMID:11843177</ref>
+
[https://www.uniprot.org/uniprot/EBP2_YEAST EBP2_YEAST] Required for the processing of the 27S pre-rRNA. Probably involved in the step of the processing of the 27 SA precursor into the 27 SB intermediate.<ref>PMID:10849420</ref> <ref>PMID:10947841</ref>
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-electron microscopy at 3.7 A resolution, revealing a half-assembled subunit. Domains I, II and VI of 25S/5.8S rRNA pack tightly into a native-like substructure, but domains III, IV and V are not assembled. The structure contains 12 assembly factors and 19 ribosomal proteins, many of which are required for early processing of large subunit rRNA. The Brx1-Ebp2 complex would interfere with the assembly of domains IV and V. Rpf1, Mak16, Nsa1 and Rrp1 form a cluster that consolidates the joining of domains I and II. Our structure reveals a key intermediate on the path to establishing the global architecture of 60S subunits.
+
-
 
+
-
Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate.,Zhou D, Zhu X, Zheng S, Tan D, Dong MQ, Ye K Protein Cell. 2018 Mar 19. pii: 10.1007/s13238-018-0526-7. doi:, 10.1007/s13238-018-0526-7. PMID:29557065<ref>PMID:29557065</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5z1g" style="background-color:#fffaf0;"></div>
+
==See Also==
==See Also==
-
*[[Ribosome biogenesis protein|Ribosome biogenesis protein]]
+
*[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Baker's yeast]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Ye, K]]
+
[[Category: Saccharomyces cerevisiae S288C]]
-
[[Category: Zheng, S]]
+
[[Category: Ye K]]
-
[[Category: Brix domain]]
+
[[Category: Zheng S]]
-
[[Category: Pre-60]]
+
-
[[Category: Pre-ribosome]]
+
-
[[Category: Ribosome]]
+

Current revision

Structure of the Brx1 and Ebp2 complex

PDB ID 5z1g

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5z1g"
Personal tools