5zx9

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Current revision (10:29, 27 March 2024) (edit) (undo)
 
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<StructureSection load='5zx9' size='340' side='right'caption='[[5zx9]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
<StructureSection load='5zx9' size='340' side='right'caption='[[5zx9]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5zx9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZX9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5zx9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZX9 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">1860c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zx9 OCA], [http://pdbe.org/5zx9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zx9 RCSB], [http://www.ebi.ac.uk/pdbsum/5zx9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zx9 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zx9 OCA], [https://pdbe.org/5zx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zx9 RCSB], [https://www.ebi.ac.uk/pdbsum/5zx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zx9 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/APA_MYCTU APA_MYCTU]] A potent antigen in animals immunized with live bacteria, it induces a strong delayed-type hypersensitivity (DTH) in immunized animals (PubMed:10531201). Elicits a mostly Th1 type of T-cell response in healthy humans; induces IFN-gamma production from CD4(+) and CD8(+) cells (PubMed:12654810). Functions as an adhesin, binds to mouse macrophages via mannose residues (PubMed:10531201, PubMed:25359607). Might interact via host CD209 (PubMed:16092920).<ref>PMID:10531201</ref> <ref>PMID:12654810</ref> <ref>PMID:16092920</ref> <ref>PMID:25359607</ref>
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[https://www.uniprot.org/uniprot/APA_MYCTU APA_MYCTU] A potent antigen in animals immunized with live bacteria, it induces a strong delayed-type hypersensitivity (DTH) in immunized animals (PubMed:10531201). Elicits a mostly Th1 type of T-cell response in healthy humans; induces IFN-gamma production from CD4(+) and CD8(+) cells (PubMed:12654810). Functions as an adhesin, binds to mouse macrophages via mannose residues (PubMed:10531201, PubMed:25359607). Might interact via host CD209 (PubMed:16092920).<ref>PMID:10531201</ref> <ref>PMID:12654810</ref> <ref>PMID:16092920</ref> <ref>PMID:25359607</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: Alanine and proline-rich protein (Apa) is a secreted antigen of Mycobacterium spp. which involves in stimulating immune responses and adhering to host cells by binding to fibronectin (Fn). Here, we report the crystal structure of Apa from Mycobacterium tuberculosis (Mtb) and its Fn-binding characteristics. METHODS: The crystal structure of Mtb Apa was determined at resolutions of 1.54A. The dissociation constants (KD) of Apa and individual modules of Fn were determined by surface plasmon resonance and enzyme-linked immunosorbent assay. Site-directed mutagenesis was performed to investigate the putative Fn-binding motif of Apa. RESULTS: Mtb Apa folds into a large seven-stranded anti-parallel beta-sheet which is flanked by three alpha-helices. The binding affinity of Mtb Apa to individual Fn modules was assessed and the results indicated that the Mtb Apa binds to FnIII-4 and FnIII-5 of Fn CBD segment. Notably, structure analysis suggested that the previously proposed Fn-binding motif (258)RWFV(261) is buried within the protein and may not be accessible to the binding counterpart. CONCLUSIONS: The structural and Fn-binding characteristics we reported here provide molecular insights into the multifunctional protein Mtb Apa. FnIII-4 and FnIII-5 of CBD are the only two modules contributing to Apa-Fn interaction. GENERAL SIGNIFICANCE: This is the first study to report the structure and Fn-binding characteristics of mycobacterial Apa. Since Apa plays a central role in stimulating immune responses and host cells adhesion, these results are of great importance in understanding the pathogenesis of mycobacterium. This information shall provide a guidance for the development of anti-mycobacteria regimen.
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Functional and structural investigations of fibronectin-binding protein Apa from Mycobacterium tuberculosis.,Kuo CJ, Gao J, Huang JW, Ko TP, Zhai C, Ma L, Liu W, Dai L, Chang YF, Chen TH, Hu Y, Yu X, Guo RT, Chen CC Biochim Biophys Acta Gen Subj. 2019 Sep;1863(9):1351-1359. doi:, 10.1016/j.bbagen.2019.06.003. Epub 2019 Jun 5. PMID:31175911<ref>PMID:31175911</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5zx9" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Myctu]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Chen, C C]]
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[[Category: Chen CC]]
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[[Category: Gao, J]]
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[[Category: Gao J]]
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[[Category: Guo, R T]]
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[[Category: Guo RT]]
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[[Category: Liu, W D]]
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[[Category: Liu WD]]
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[[Category: Cyclolavandulyl diphosphate synthase]]
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[[Category: Inhibitor]]
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[[Category: Protein binding]]
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[[Category: Substrate binding]]
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Current revision

Crystal structure of apo form fibronectin-binding protein Apa from Mycobacterium tuberculosis

PDB ID 5zx9

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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