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| | ==Structure of Ctr9, Paf1 and Cdc73 ternary complex from Myceliophthora thermophila== | | ==Structure of Ctr9, Paf1 and Cdc73 ternary complex from Myceliophthora thermophila== |
| - | <StructureSection load='6af0' size='340' side='right' caption='[[6af0]], [[Resolution|resolution]] 2.88Å' scene=''> | + | <StructureSection load='6af0' size='340' side='right'caption='[[6af0]], [[Resolution|resolution]] 2.88Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6af0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctt Myctt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AF0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AF0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6af0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermothelomyces_thermophilus_ATCC_42464 Thermothelomyces thermophilus ATCC 42464]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AF0 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MYCTH_2305158 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573729 MYCTT]), MYCTH_2303763 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573729 MYCTT]), MYCTH_2298931 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573729 MYCTT])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.88Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6af0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6af0 OCA], [http://pdbe.org/6af0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6af0 RCSB], [http://www.ebi.ac.uk/pdbsum/6af0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6af0 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6af0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6af0 OCA], [https://pdbe.org/6af0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6af0 RCSB], [https://www.ebi.ac.uk/pdbsum/6af0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6af0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/G2QC65_MYCTT G2QC65_MYCTT] |
| - | The polymerase-associated factor 1 (Paf1) complex is a general transcription elongation factor of RNA polymerase II, which is composed of five core subunits, Paf1, Ctr9, Cdc73, Leo1, and Rtf1, and functions as a diverse platform that broadly affects gene expression genome-wide. In this study, we solved the 2.9-A crystal structure of the core region composed of the Ctr9-Paf1-Cdc73 ternary complex from a thermophilic fungi, which provides a structural perspective of the molecular details of the organization and interactions involving the Paf1 subunits in the core complex. We find that Ctr9 is composed of 21 tetratricopeptide repeat (TPR) motifs that wrap three circular turns in a right-handed superhelical manner around the N-terminal region of an elongated single-polypeptide-chain scaffold of Paf1. The Cdc73 fragment is positioned within the surface groove of Ctr9, where it contacts mainly with Ctr9 and minimally with Paf1. We also identified that the Paf1 complex preferentially binds single-strand-containing DNAs. Our work provides structural insights into the overall architecture of the Paf1 complex and paves the road forward for understanding the molecular mechanisms of the Paf1 complex in transcriptional regulation.
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| - | Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology.,Deng P, Zhou Y, Jiang J, Li H, Tian W, Cao Y, Qin Y, Kim J, Roeder RG, Patel DJ, Wang Z Proc Natl Acad Sci U S A. 2018 Oct 2;115(40):9998-10003. doi:, 10.1073/pnas.1812256115. Epub 2018 Sep 17. PMID:30224485<ref>PMID:30224485</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 6af0" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Myctt]] | + | [[Category: Large Structures]] |
| - | [[Category: Deng, P]] | + | [[Category: Thermothelomyces thermophilus ATCC 42464]] |
| - | [[Category: Wang, Z]] | + | [[Category: Deng P]] |
| - | [[Category: Zhou, Y]] | + | [[Category: Wang Z]] |
| - | [[Category: Cdc73]] | + | [[Category: Zhou Y]] |
| - | [[Category: Crystal structure]]
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| - | [[Category: Ctr9]]
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| - | [[Category: Paf1]]
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| - | [[Category: Transcription]]
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| - | [[Category: Transcription elongation]]
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