6agz

<table><tr><td colspan='2'>[[6agz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AGZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AGZ FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6agz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6agz OCA], [http://pdbe.org/6agz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6agz RCSB], [http://www.ebi.ac.uk/pdbsum/6agz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6agz ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Old yellow enzymes (OYEs) are potential targets of protein engineering for useful biocatalysts because of their excellent asymmetric reductions of enone compounds. Two OYEs from different yeast strains, Candida macedoniensis AKU4588 OYE (CmOYE) and Pichia sp. AKU4542 OYE (PsOYE), have a sequence identity of 46%, but show different substrate preferences; PsOYE shows 3.4-fold and 39-fold higher catalytic activities than CmOYE toward ketoisophorone and (4S)-phorenol, respectively. To gain insights into structural basis of their different substrate preferences, we have solved a crystal structure of PsOYE, and compared its catalytic site structure with that of CmOYE, revealing the catalytic pocket of PsOYE is wider than that of CmOYE due to different positions of Phe(246) (PsOYE)/Phe(250) (CmOYE) in static Loop 5. This study shows a significance of 3D structural information to explain the different substrate preferences of yeast OYEs which cannot be understood from their amino acid sequences. Abbreviations: OYE: Old yellow enzymes, CmOYE: Candida macedoniensis AKU4588 OYE, PsOYE: Pichia sp. AKU4542 OYE.

 

== References ==

[[Category: Horita, S]]

[[Category: Kataoka, M]]

[[Category: Kitamura, N]]

[[Category: Miyakawa, T]]

[[Category: Nagata, K]]

[[Category: Nakagawa, T]]

[[Category: Ohtsuka, J]]

[[Category: Shimizu, S]]

[[Category: Tanokura, M]]

[[Category: Tim barrel motif]]