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| | <StructureSection load='6aho' size='340' side='right'caption='[[6aho]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='6aho' size='340' side='right'caption='[[6aho]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6aho]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AHO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6AHO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6aho]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AHO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AHO FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KAP114, YGL241W, HRC1004 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.502Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6aho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aho OCA], [http://pdbe.org/6aho PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6aho RCSB], [http://www.ebi.ac.uk/pdbsum/6aho PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6aho ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6aho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aho OCA], [https://pdbe.org/6aho PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6aho RCSB], [https://www.ebi.ac.uk/pdbsum/6aho PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6aho ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/IMB5_YEAST IMB5_YEAST]] Required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Serves a receptor for nuclear localization signals. Mediates the nuclear import of TATA-binding protein (TBP) and of histones H2A and H2B.<ref>PMID:10535958</ref> <ref>PMID:11309407</ref> | + | [https://www.uniprot.org/uniprot/IMB5_YEAST IMB5_YEAST] Required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Serves a receptor for nuclear localization signals. Mediates the nuclear import of TATA-binding protein (TBP) and of histones H2A and H2B.<ref>PMID:10535958</ref> <ref>PMID:11309407</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Nuclear accessibility of transcription factors controls gene expression, co-regulated by Ran-dependent nuclear localization and a competitive regulatory network. Here, we reveal that nuclear import factor-facilitated transcriptional repression attenuates ribosome biogenesis under chronic salt stress. Kap114p, one of the karyopherin-betas (Kap-betas) that mediates nuclear import of yeast TATA-binding protein (yTBP), exhibits a yTBP-binding affinity four orders of magnitude greater than its counterparts and suppresses binding of yTBP with DNA. Our crystal structure of Kap114p reveals an extensively negatively charged concave surface, accounting for high-affinity basic-protein binding. KAP114 knockout in yeast leads to a high-salt growth defect, with transcriptomic analyses revealing that Kap114p modulates expression of genes associated with ribosomal biogenesis by suppressing yTBP binding to target promoters, a trans-repression mechanism we attribute to reduced nuclear Ran levels under salinity stress. Our findings reveal that Ran integrates the nuclear transport pathway and transcription regulatory network, allowing yeast to respond to environmental stresses.
| + | |
| | | | |
| - | Karyopherin Kap114p-mediated trans-repression controls ribosomal gene expression under saline stress.,Liao CC, Shankar S, Pi WC, Chang CC, Ahmed GR, Chen WY, Hsia KC EMBO Rep. 2020 Jun 2:e48324. doi: 10.15252/embr.201948324. PMID:32484313<ref>PMID:32484313</ref>
| + | ==See Also== |
| - | | + | *[[Importin 3D structures|Importin 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6aho" style="background-color:#fffaf0;"></div>
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ahmed, G R]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Hsia, K C]] | + | [[Category: Ahmed GR]] |
| - | [[Category: Liao, C C]] | + | [[Category: Hsia KC]] |
| - | [[Category: Shankar, S]] | + | [[Category: Liao CC]] |
| - | [[Category: Karyopherin]] | + | [[Category: Shankar S]] |
| - | [[Category: Nuclear transport]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Tata box binding protein]]
| + | |
| - | [[Category: Transcription]]
| + | |
