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6ifb

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Current revision (10:33, 27 March 2024) (edit) (undo)
 
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<StructureSection load='6ifb' size='340' side='right'caption='[[6ifb]], [[Resolution|resolution]] 1.37&Aring;' scene=''>
<StructureSection load='6ifb' size='340' side='right'caption='[[6ifb]], [[Resolution|resolution]] 1.37&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6ifb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica_hm-1:imss Entamoeba histolytica hm-1:imss]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IFB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6IFB FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6ifb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica_HM-1:IMSS Entamoeba histolytica HM-1:IMSS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IFB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IFB FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=RM4:6-DEOXY-BETA-L-MANNOPYRANOSE'>RM4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.37&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ifb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ifb OCA], [http://pdbe.org/6ifb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ifb RCSB], [http://www.ebi.ac.uk/pdbsum/6ifb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ifb ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=RM4:6-DEOXY-BETA-L-MANNOPYRANOSE'>RM4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ifb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ifb OCA], [https://pdbe.org/6ifb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ifb RCSB], [https://www.ebi.ac.uk/pdbsum/6ifb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ifb ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/N9TFI9_ENTH1 N9TFI9_ENTH1]
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beta-trefoil lectins are galactose/N-acetyl galactosamine specific lectins, which are widely distributed across all kingdoms of life and are known to perform several important functions. However, there is no report available on the characterization of these lectins from protozoans. We have performed structural and biophysical studies on a beta-trefoil lectin from Entamoeba histolytica (EntTref) which exists as a mixture of monomers and dimers in solution. Further, we have determined the affinities of EntTref for rhamnose, galactose and different galactose-linked sugars. We obtained the crystal structure of EntTref in a sugar-free form (EntTref_apo) and a rhamnose-bound form (EntTref_rham). A novel Cys residue-mediated dimerization was revealed in the crystal structure of EntTref_apo while the structure of EntTref_rham provided the structural basis for the recognition of rhamnose by a beta-trefoil lectin for the first time. To the best of our knowledge, this is the only report of the structural, functional and biophysical characterization of a beta-trefoil lectin from a protozoan source and the first report of Cys-mediated dimerization in this class of lectins.
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Crystal structures of a beta-trefoil lectin from Entamoeba histolytica in monomeric and a novel disulphide bond-mediated dimeric forms.,Khan F, Kurre D, Suguna K Glycobiology. 2020 Jan 21. pii: 5710858. doi: 10.1093/glycob/cwaa001. PMID:31967310<ref>PMID:31967310</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6ifb" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Entamoeba histolytica hm-1:imss]]
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[[Category: Entamoeba histolytica HM-1:IMSS]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Khan, F]]
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[[Category: Khan F]]
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[[Category: Suguna, K]]
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[[Category: Suguna K]]
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[[Category: Beta-trefoil]]
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[[Category: Entamoeba histolytica]]
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[[Category: Lectin]]
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[[Category: Rhamnose]]
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[[Category: Sugar binding protein]]
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Current revision

Structure of rhamnose bound beta-trefoil lectin from Entamoeba histolytica

PDB ID 6ifb

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