This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


6ifw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:33, 27 March 2024) (edit) (undo)
 
Line 1: Line 1:
==crystal structure of chimeric construct of KsgA with loop 1 from erm==
==crystal structure of chimeric construct of KsgA with loop 1 from erm==
-
<StructureSection load='6ifw' size='340' side='right' caption='[[6ifw]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
+
<StructureSection load='6ifw' size='340' side='right'caption='[[6ifw]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[6ifw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IFW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IFW FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[6ifw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IFW FirstGlance]. <br>
-
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rsmA, ksgA, BSU00420 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
-
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/16S_rRNA_(adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.182 2.1.1.182] </span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ifw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ifw OCA], [https://pdbe.org/6ifw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ifw RCSB], [https://www.ebi.ac.uk/pdbsum/6ifw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ifw ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ifw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ifw OCA], [http://pdbe.org/6ifw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ifw RCSB], [http://www.ebi.ac.uk/pdbsum/6ifw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ifw ProSAT]</span></td></tr>
+
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/RSMA_BACSU RSMA_BACSU]] Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.[HAMAP-Rule:MF_00607]
+
[https://www.uniprot.org/uniprot/RSMA_BACSU RSMA_BACSU] Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.[HAMAP-Rule:MF_00607]
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Post-translational methylation of ribosomal RNA at select positions is a prevalent resistance mechanism adopted by pathogens. In this work, KsgA, a housekeeping ribosomal methyltransferase (rMtase) involved in ribosome biogenesis, was exploited as a model system to delineate the specific targeting determinants that impart substrate specificity to rMtases. With a combination of evolutionary and structure-guided approaches, a set of chimeras were created that altered the targeting specificity of KsgA such that it acted similarly to erythromycin-resistant methyltransferases (Erms), rMtases found in multidrug-resistant pathogens. The results revealed that specific loop embellishments on the basic Rossmann fold are key determinants in the selection of the cognate RNA. Moreover, in vivo studies confirmed that chimeric constructs are competent in imparting macrolide resistance. This work explores the factors that govern the emergence of resistance and paves the way for the design of specific inhibitors useful in reversing antibiotic resistance.
+
-
Deciphering Determinants in Ribosomal Methyltransferases that Confer Antimicrobial Resistance.,Bhujbalrao R, Anand R J Am Chem Soc. 2019 Jan 9. doi: 10.1021/jacs.8b10277. PMID:30624914<ref>PMID:30624914</ref>
+
==See Also==
-
 
+
*[[Adenosine dimethyltransferase 3D structures|Adenosine dimethyltransferase 3D structures]]
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 6ifw" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Bacsu]]
+
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
-
[[Category: Anand, R]]
+
[[Category: Large Structures]]
-
[[Category: Bhujbalrao, R]]
+
[[Category: Anand R]]
-
[[Category: Chimera]]
+
[[Category: Bhujbalrao R]]
-
[[Category: Erythromycin]]
+
-
[[Category: Resistance]]
+
-
[[Category: Transferase]]
+

Current revision

crystal structure of chimeric construct of KsgA with loop 1 from erm

PDB ID 6ifw

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6ifw"
Personal tools