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| | <StructureSection load='6imp' size='340' side='right'caption='[[6imp]], [[Resolution|resolution]] 2.62Å' scene=''> | | <StructureSection load='6imp' size='340' side='right'caption='[[6imp]], [[Resolution|resolution]] 2.62Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6imp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibvm Vibvm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IMP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IMP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6imp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_vulnificus_MO6-24/O Vibrio vulnificus MO6-24/O]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IMP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IMP FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6imp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6imp OCA], [http://pdbe.org/6imp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6imp RCSB], [http://www.ebi.ac.uk/pdbsum/6imp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6imp ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.62Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6imp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6imp OCA], [https://pdbe.org/6imp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6imp RCSB], [https://www.ebi.ac.uk/pdbsum/6imp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6imp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A3Q0KY79_VIBVU A0A3Q0KY79_VIBVU] |
| - | Upon invading target cells, multifunctional autoprocessing repeats-in-toxin (MARTX) toxins secreted by bacterial pathogens release their disease-related modularly structured effector domains. However, it is unclear how a diverse repertoire of effector domains within these toxins are processed and activated. Here, we report that Makes caterpillars floppy-like effector (MCF)-containing MARTX toxins require ubiquitous ADP-ribosylation factor (ARF) proteins for processing and activation of intermediate effector modules, which localize in different subcellular compartments following limited processing of holo effector modules by the internal cysteine protease. Effector domains structured tandemly with MCF in intermediate modules become disengaged and fully activated by MCF, which aggressively interacts with ARF proteins present at the same location as intermediate modules and is converted allosterically into a catalytically competent protease. MCF-mediated effector processing leads ultimately to severe virulence in mice via an MCF-mediated ARF switching mechanism across subcellular compartments. This work provides insight into how bacteria take advantage of host systems to induce systemic pathogenicity.
| + | |
| - | | + | |
| - | Makes caterpillars floppy-like effector-containing MARTX toxins require host ADP-ribosylation factor (ARF) proteins for systemic pathogenicity.,Lee Y, Kim BS, Choi S, Lee EY, Park S, Hwang J, Kwon Y, Hyun J, Lee C, Kim JF, Eom SH, Kim MH Proc Natl Acad Sci U S A. 2019 Sep 3;116(36):18031-18040. doi:, 10.1073/pnas.1905095116. Epub 2019 Aug 19. PMID:31427506<ref>PMID:31427506</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6imp" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Vibvm]] | + | [[Category: Vibrio vulnificus MO6-24/O]] |
| - | [[Category: Hwang, J]] | + | [[Category: Hwang J]] |
| - | [[Category: Kim, M H]] | + | [[Category: Kim MH]] |
| - | [[Category: Alpha-beta fold]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Virulence]]
| + | |
