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6ixw

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Current revision (10:35, 27 March 2024) (edit) (undo)
 
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<StructureSection load='6ixw' size='340' side='right'caption='[[6ixw]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
<StructureSection load='6ixw' size='340' side='right'caption='[[6ixw]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6ixw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_prevot_594 Clostridium botulinum prevot_594]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IXW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IXW FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6ixw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum_Prevot_594 Clostridium botulinum Prevot_594]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IXW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IXW FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.253&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">T258_3831 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1408284 Clostridium botulinum Prevot_594])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ixw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ixw OCA], [http://pdbe.org/6ixw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ixw RCSB], [http://www.ebi.ac.uk/pdbsum/6ixw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ixw ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ixw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ixw OCA], [https://pdbe.org/6ixw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ixw RCSB], [https://www.ebi.ac.uk/pdbsum/6ixw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ixw ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A0B4W229_CLOBO A0A0B4W229_CLOBO]
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Microfilaments (actin) and microtubules represent the extremes in eukaryotic cytoskeleton cross-sectional dimensions, raising the question of whether filament architectures are limited by protein fold. Here, we report the cryoelectron microscopy structure of a complex filament formed from 15 protofilaments of an actin-like protein. This actin-like ParM is encoded on the large pCBH Clostridium botulinum plasmid. In cross-section, the ~26 nm diameter filament comprises a central helical protofilament surrounded by intermediate and outer layers of six and eight twisted protofilaments, respectively. Alternating polarity of the layers allows for similar lateral contacts between each layer. This filament design is stiffer than the actin filament, and has likely been selected for during evolution to move large cargos. The comparable sizes of microtubule and pCBH ParM filaments indicate that larger filament architectures are not limited by the protomer fold. Instead, function appears to have been the evolutionary driving force to produce broad, complex filaments.
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The structure of a 15-stranded actin-like filament from Clostridium botulinum.,Koh F, Narita A, Lee LJ, Tanaka K, Tan YZ, Dandey VP, Popp D, Robinson RC Nat Commun. 2019 Jun 28;10(1):2856. doi: 10.1038/s41467-019-10779-9. PMID:31253774<ref>PMID:31253774</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6ixw" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Clostridium botulinum prevot_594]]
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[[Category: Clostridium botulinum Prevot_594]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Koh, F]]
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[[Category: Koh F]]
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[[Category: Narita, A]]
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[[Category: Narita A]]
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[[Category: Popp, D]]
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[[Category: Popp D]]
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[[Category: Robinson, R C]]
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[[Category: Robinson RC]]
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[[Category: Actin parm]]
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[[Category: Structural protein]]
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Current revision

pCBH ParM non-polymerisable quadruple mutant

PDB ID 6ixw

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