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| | <SX load='6j7v' size='340' side='right' viewer='molstar' caption='[[6j7v]], [[Resolution|resolution]] 16.00Å' scene=''> | | <SX load='6j7v' size='340' side='right' viewer='molstar' caption='[[6j7v]], [[Resolution|resolution]] 16.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6j7v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Halorubrum_pleomorphic_virus_6 Halorubrum pleomorphic virus 6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J7V OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6J7V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6j7v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halorubrum_pleomorphic_virus_6 Halorubrum pleomorphic virus 6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J7V FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6q6l|6q6l]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 16Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6j7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j7v OCA], [http://pdbe.org/6j7v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j7v RCSB], [http://www.ebi.ac.uk/pdbsum/6j7v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j7v ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j7v OCA], [https://pdbe.org/6j7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j7v RCSB], [https://www.ebi.ac.uk/pdbsum/6j7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j7v ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/H9ABP6_9VIRU H9ABP6_9VIRU] |
| - | Lipid membrane fusion is an essential function in many biological processes. Detailed mechanisms of membrane fusion and the protein structures involved have been mainly studied in eukaryotic systems, whereas very little is known about membrane fusion in prokaryotes. Haloarchaeal pleomorphic viruses (HRPVs) have a membrane envelope decorated with spikes that are presumed to be responsible for host attachment and membrane fusion. Here we determine atomic structures of the ectodomains of the 57-kDa spike protein VP5 from two related HRPVs revealing a previously unreported V-shaped fold. By Volta phase plate cryo-electron tomography we show that VP5 is monomeric on the viral surface, and we establish the orientation of the molecules with respect to the viral membrane. We also show that the viral membrane fuses with the host cytoplasmic membrane in a process mediated by VP5. This sheds light on protein structures involved in prokaryotic membrane fusion.
| + | |
| - | | + | |
| - | The structure of a prokaryotic viral envelope protein expands the landscape of membrane fusion proteins.,El Omari K, Li S, Kotecha A, Walter TS, Bignon EA, Harlos K, Somerharju P, De Haas F, Clare DK, Molin M, Hurtado F, Li M, Grimes JM, Bamford DH, Tischler ND, Huiskonen JT, Stuart DI, Roine E Nat Commun. 2019 Feb 19;10(1):846. doi: 10.1038/s41467-019-08728-7. PMID:30783086<ref>PMID:30783086</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6j7v" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| | [[Category: Halorubrum pleomorphic virus 6]] | | [[Category: Halorubrum pleomorphic virus 6]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Huiskonen, J T]] | + | [[Category: El Omari K]] |
| - | [[Category: Li, S]]
| + | [[Category: Huiskonen JT]] |
| - | [[Category: Omari, K El]]
| + | [[Category: Li S]] |
| - | [[Category: Stuart, D I]] | + | [[Category: Stuart DI]] |
| - | [[Category: Archaea]] | + | |
| - | [[Category: Envelope protein]] | + | |
| - | [[Category: Fusion protein]]
| + | |
| - | [[Category: Haloarchaea]]
| + | |
| - | [[Category: Hrpv6]]
| + | |
| - | [[Category: Spike]]
| + | |
| - | [[Category: Viral protein]]
| + | |
