6jd7

<table><tr><td colspan='2'>[[6jd7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Neim8 Neim8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JD7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6JD7 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CIJ84_02100 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=604162 NEIM8])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jd7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jd7 OCA], [http://pdbe.org/6jd7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jd7 RCSB], [http://www.ebi.ac.uk/pdbsum/6jd7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jd7 ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

CRISPR-Cas adaptive immune systems function to protect bacteria from invasion by foreign genetic elements. The CRISPR-Cas9 system has been widely adopted as a powerful genome-editing tool, and phage-encoded inhibitors, known as anti-CRISPRs, offer a means of regulating its activity. Here, we report the crystal structures of anti-CRISPR protein AcrIIC2Nme alone and in complex with Nme1Cas9. We demonstrate that AcrIIC2Nme inhibits Cas9 through interactions with the positively charged bridge helix, thereby preventing sgRNA loading. In vivo phage plaque assays and in vitro DNA cleavage assays show that AcrIIC2Nme mediates its activity through a large electronegative surface. This work shows that anti-CRISPR activity can be mediated through the inhibition of Cas9 complex assembly.

Inhibition of CRISPR-Cas9 ribonucleoprotein complex assembly by anti-CRISPR AcrIIC2.,Thavalingam A, Cheng Z, Garcia B, Huang X, Shah M, Sun W, Wang M, Harrington L, Hwang S, Hidalgo-Reyes Y, Sontheimer EJ, Doudna J, Davidson AR, Moraes TF, Wang Y, Maxwell KL Nat Commun. 2019 Jun 26;10(1):2806. doi: 10.1038/s41467-019-10577-3. PMID:31243272<ref>PMID:31243272</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 6jd7" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Neim8]]

[[Category: Cheng, Z]]

[[Category: Huang, X]]

[[Category: Sun, W]]

[[Category: Wang, Y L]]

[[Category: Nmecas9]]