6jg3

<table><tr><td colspan='2'>[[6jg3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JG3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6JG3 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jg3 OCA], [http://pdbe.org/6jg3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jg3 RCSB], [http://www.ebi.ac.uk/pdbsum/6jg3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jg3 ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

The type 2 ryanodine receptor (RyR2) is responsible for releasing Ca(2+) from the sarcoplasmic reticulum of cardiomyocytes, subsequently leading to muscle contraction. Here, we report 4 cryo-electron microscopy (cryo-EM) structures of porcine RyR2 bound to distinct modulators that, together with our published structures, provide mechanistic insight into RyR2 regulation. Ca(2+) alone induces a contraction of the central domain that facilitates the dilation of the S6 bundle but is insufficient to open the pore. The small-molecule agonist PCB95 helps Ca(2+) to overcome the barrier for opening. FKBP12.6 induces a relaxation of the central domain that decouples it from the S6 bundle, stabilizing RyR2 in a closed state even in the presence of Ca(2+) and PCB95. Although the channel is open when PCB95 is replaced by caffeine and adenosine 5'-triphosphate (ATP), neither of the modulators alone can sufficiently counter the antagonistic effect to open the channel. Our study marks an important step toward mechanistic understanding of the sophisticated regulation of this key channel whose aberrant activity engenders life-threatening cardiac disorders.

 

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== References ==

[[Category: Chi, X M]]

[[Category: Gong, D S]]

[[Category: Huang, G X.Y]]

[[Category: Lei, J L]]

[[Category: Ren, K]]

[[Category: Yan, N]]

[[Category: Zhou, G W]]

[[Category: Zhou, Q]]

[[Category: Membrane protein]]