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| | <StructureSection load='6jom' size='340' side='right'caption='[[6jom]], [[Resolution|resolution]] 2.45Å' scene=''> | | <StructureSection load='6jom' size='340' side='right'caption='[[6jom]], [[Resolution|resolution]] 2.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6jom]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mychj Mychj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JOM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6JOM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6jom]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycoplasma_hyopneumoniae_J Mycoplasma hyopneumoniae J]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JOM FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LAQ:5-O-[(R)-({5-[(3R)-1,2-DITHIOLAN-3-YL]PENTANOYL}OXY)(HYDROXY)PHOSPHORYL]ADENOSINE'>LAQ</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lplA-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262719 MYCHJ])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LAQ:5-O-[(R)-({5-[(3R)-1,2-DITHIOLAN-3-YL]PENTANOYL}OXY)(HYDROXY)PHOSPHORYL]ADENOSINE'>LAQ</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lipoate--protein_ligase Lipoate--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.20 6.3.1.20] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jom OCA], [https://pdbe.org/6jom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jom RCSB], [https://www.ebi.ac.uk/pdbsum/6jom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jom ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jom OCA], [http://pdbe.org/6jom PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jom RCSB], [http://www.ebi.ac.uk/pdbsum/6jom PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jom ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q4AA29_MESHJ Q4AA29_MESHJ] |
| - | Mycoplasma hyopneumoniae (M. hyopneumoniae) is the causative agent of pandemic pneumonia among pigs, namely, swine enzootic pneumonia. Although M. hyopneumoniae was first identified in 1965, little is known regarding its metabolic pathways, which might play a pivotal role during disease pathogenesis. Lipoate is an essential cofactor for enzymes important for central metabolism. However, the lipoate metabolism pathway in M. hyopneumoniae is definitely unclear. Here, we identified a novel gene, lpl, encoding a lipoate protein ligase in the genome of M. hyopneumoniae (Mhp-Lpl). This gene contains 1,032 base pairs and encodes a protein of 343 amino acids, which is between 7.5 and 36.09% identical to lipoate protein ligases (Lpls) of other species. Similar to its homologs in other species, Mhp-Lpl catalyzes the ATP-dependent activation of lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of M. hyopneumoniae GcvH (Mhp H) in vitro. Enzymatic and mutagenesis analysis indicate that residue K56 within the SKT sequence of Mhp H protein is the lipoyl moiety acceptor site. The three-dimensional structure showed typical lipoate protein ligase folding, with a large N-terminal domain and a small C-terminal domain. The large N-terminal domain is responsible for the full enzymatic activity of Mhp-Lpl. The identification and characterization of Mhp-Lpl will be beneficial to our understanding of M. hyopneumoniae metabolism. Summary: Lipoic acid is an essential cofactor for the activation of some enzyme complexes involved in key metabolic processes. Lipoate protein ligases (Lpls) are responsible for the metabolism of lipoic acid. To date, little is known regarding the Lpls in M. hyopneumoniae. In this study, we identified a lipoate protein ligase of M. hyopneumoniae. We further analyzed the function, overall structure and ligand-binding site of this protein. The lipoate acceptor site on M. hyopneumoniae GcvH was also identified. Together, these findings reveal that Lpl exists in M. hyopneumoniae and will provide a basis for further exploration of the pathway of lipoic acid metabolism in M. hyopneumoniae.
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| - | Functional Identification and Structural Analysis of a New Lipoate Protein Ligase in Mycoplasma hyopneumoniae.,Zhu K, Chen H, Jin J, Wang N, Ma G, Huang J, Feng Y, Xin J, Zhang H, Liu H Front Cell Infect Microbiol. 2020 Apr 21;10:156. doi: 10.3389/fcimb.2020.00156., eCollection 2020. PMID:32373550<ref>PMID:32373550</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6jom" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lipoate--protein ligase]] | + | [[Category: Mycoplasma hyopneumoniae J]] |
| - | [[Category: Mychj]]
| + | [[Category: Chen H]] |
| - | [[Category: Chen, H]] | + | [[Category: Ma G]] |
| - | [[Category: Ma, G]] | + | [[Category: Zhang H]] |
| - | [[Category: Zhang, H]] | + | |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Lipoate protein ligase]]
| + | |
| - | [[Category: Lipoyl-amp]]
| + | |
| - | [[Category: Lpl]]
| + | |
| - | [[Category: Mycoplasma hyopneumoniae]]
| + | |
