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6jtd

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Current revision (10:39, 27 March 2024) (edit) (undo)
 
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<StructureSection load='6jtd' size='340' side='right'caption='[[6jtd]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
<StructureSection load='6jtd' size='340' side='right'caption='[[6jtd]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6jtd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trollius_chinensis Trollius chinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JTD FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6jtd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trollius_chinensis Trollius chinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JTD FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtd OCA], [http://pdbe.org/6jtd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jtd RCSB], [http://www.ebi.ac.uk/pdbsum/6jtd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtd ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtd OCA], [https://pdbe.org/6jtd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jtd RCSB], [https://www.ebi.ac.uk/pdbsum/6jtd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtd ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A4Y5RXX8_9MAGN A0A4Y5RXX8_9MAGN]
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In this work, we explored the catalytic promiscuity of TcCGT1, a new C-glycosyltransferase (CGT) from the medicinal plant Trollius chinensis. TcCGT1 could efficiently and regio-specifically catalyze 8-C-glycosylation of 36 flavones and other flavonoids, and could also catalyze the O-glycosylation of diverse phenolics. Moreover, the crystal structure of TcCGT1 in complex with uridine diphosphate was determined at 1.85 A resolution. Structural analysis with molecular docking revealed a new model for catalytic mechanism of TcCGT1, which was initiated by substrate spontaneous deprotonation. The spacious binding pocket explains the robust substrate promiscuity, and binding pose of the substrate determines C- or O-glycosylation activity. Site-directed mutagenesis at two residues (I94E and G284K) switched C- to O-glycosylation. This work highlights TcCGT1 as the first plant CGT with a crystal structure and the first flavone 8-C-glycosyltransferase, and provides a basis for protein engineering to design efficient glycosylation biocatalysts for drug discovery.
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Molecular Characterization and Structural Basis of a Promiscuous C-Glycosyltransferase from Trollius chinensis.,He JB, Zhao P, Hu ZM, Liu S, Kuang Y, Zhang M, Li B, Yun CH, Qiao X, Ye M Angew Chem Int Ed Engl. 2019 Jun 4. doi: 10.1002/anie.201905505. PMID:31163097<ref>PMID:31163097</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6jtd" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Trollius chinensis]]
[[Category: Trollius chinensis]]
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[[Category: Yun, C H]]
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[[Category: Yun CH]]
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[[Category: Zhao, P]]
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[[Category: Zhao P]]
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[[Category: C-glycosyltransferase]]
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[[Category: Glycosyltransferase]]
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[[Category: Transferase]]
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Current revision

Crystal structure of TcCGT1 in complex with UDP

PDB ID 6jtd

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