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| | <SX load='6jy0' size='340' side='right' viewer='molstar' caption='[[6jy0]], [[Resolution|resolution]] 3.56Å' scene=''> | | <SX load='6jy0' size='340' side='right' viewer='molstar' caption='[[6jy0]], [[Resolution|resolution]] 3.56Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6jy0]] is a 22 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JY0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6JY0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6jy0]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JY0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JY0 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fljB, C2253_13415, CET98_21075, D7F20_13880, D7H43_12410, DJ388_21755 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.56Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jy0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jy0 OCA], [http://pdbe.org/6jy0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jy0 RCSB], [http://www.ebi.ac.uk/pdbsum/6jy0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jy0 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jy0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jy0 OCA], [https://pdbe.org/6jy0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jy0 RCSB], [https://www.ebi.ac.uk/pdbsum/6jy0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jy0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/Q549S3_SALTM Q549S3_SALTM]] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.[RuleBase:RU362073] | + | [https://www.uniprot.org/uniprot/Q549S3_SALTM Q549S3_SALTM] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.[RuleBase:RU362073] |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The bacterial flagellum is a motility organelle consisting of a long helical filament as a propeller and a rotary motor that drives rapid filament rotation to produce thrust. Salmonella enterica serovar Typhimurium has two genes of flagellin, fljB and fliC, for flagellar filament formation and autonomously switches their expression at a frequency of 10(-3)-10(-4) per cell per generation. We report here differences in their structures and motility functions under high-viscosity conditions. A Salmonella strain expressing FljB showed a higher motility than one expressing FliC under high viscosity. To examine the reasons for this motility difference, we carried out structural analyses of the FljB filament by electron cryomicroscopy and found that the structure was nearly identical to that of the FliC filament except for the position and orientation of the outermost domain D3 of flagellin. The density of domain D3 was much lower in FljB than FliC, suggesting that domain D3 of FljB is more flexible and mobile than that of FliC. These differences suggest that domain D3 plays an important role not only in changing antigenicity of the filament but also in optimizing motility function of the filament as a propeller under different conditions.
| + | |
| | | | |
| - | Structural and Functional Comparison of Salmonella Flagellar Filaments Composed of FljB and FliC.,Yamaguchi T, Toma S, Terahara N, Miyata T, Ashihara M, Minamino T, Namba K, Kato T Biomolecules. 2020 Feb 6;10(2). pii: biom10020246. doi: 10.3390/biom10020246. PMID:32041169<ref>PMID:32041169</ref>
| + | ==See Also== |
| - | | + | *[[Flagellin 3D structures|Flagellin 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6jy0" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Bacillus typhimurium loeffler 1892]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ashikara, M]] | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium]] |
| - | [[Category: Kato, T]] | + | [[Category: Ashikara M]] |
| - | [[Category: Minamino, T]] | + | [[Category: Kato T]] |
| - | [[Category: Miyata, T]] | + | [[Category: Minamino T]] |
| - | [[Category: Namba, K]] | + | [[Category: Miyata T]] |
| - | [[Category: Terahara, N]] | + | [[Category: Namba K]] |
| - | [[Category: Toma, S]] | + | [[Category: Terahara N]] |
| - | [[Category: Yamaguchi, T]] | + | [[Category: Toma S]] |
| - | [[Category: Flagellar motor]]
| + | [[Category: Yamaguchi T]] |
| - | [[Category: Fljb]]
| + | |
| - | [[Category: Helical reconstruction]]
| + | |
| - | [[Category: Protein fibril]]
| + | |
| - | [[Category: Salmonella]]
| + | |
