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| | <SX load='6k0b' size='340' side='right' viewer='molstar' caption='[[6k0b]], [[Resolution|resolution]] 4.30Å' scene=''> | | <SX load='6k0b' size='340' side='right' viewer='molstar' caption='[[6k0b]], [[Resolution|resolution]] 4.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6k0b]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Metja Metja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K0B OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K0B FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6k0b]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] and [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K0B FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6k0a|6k0a]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rnp2, MJ0494 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA]), rnp3, MJ1139 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA]), rnp1, MJ0464 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA]), rnp4, MJ0962 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA]), rpl7ae, MJ1203 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k0b OCA], [https://pdbe.org/6k0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k0b RCSB], [https://www.ebi.ac.uk/pdbsum/6k0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k0b ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_P Ribonuclease P], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.5 3.1.26.5] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k0b OCA], [http://pdbe.org/6k0b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k0b RCSB], [http://www.ebi.ac.uk/pdbsum/6k0b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k0b ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RNP4_METJA RNP4_METJA]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00757]<ref>PMID:18558617</ref> <ref>PMID:21683084</ref> <ref>PMID:22298511</ref> [[http://www.uniprot.org/uniprot/RNP2_METJA RNP2_METJA]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00755]<ref>PMID:18558617</ref> <ref>PMID:21683084</ref> <ref>PMID:22298511</ref> [[http://www.uniprot.org/uniprot/RNP1_METJA RNP1_METJA]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00754]<ref>PMID:18558617</ref> <ref>PMID:21683084</ref> <ref>PMID:22298511</ref> [[http://www.uniprot.org/uniprot/RNP3_METJA RNP3_METJA]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00756]<ref>PMID:18558617</ref> <ref>PMID:21683084</ref> <ref>PMID:22298511</ref> [[http://www.uniprot.org/uniprot/RL7A_METJA RL7A_METJA]] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs. | + | [https://www.uniprot.org/uniprot/RL7A_METJA RL7A_METJA] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Ribonuclease P (RNase P) is an essential ribozyme responsible for tRNA 5' maturation. Here we report the cryo-EM structures of Methanocaldococcus jannaschii (Mja) RNase P holoenzyme alone and in complex with a tRNA substrate at resolutions of 4.6 A and 4.3 A, respectively. The structures reveal that the subunits of MjaRNase P are strung together to organize the holoenzyme in a dimeric conformation required for efficient catalysis. The structures also show that archaeal RNase P is a functional chimera of bacterial and eukaryal RNase Ps that possesses bacterial-like two RNA-based anchors and a eukaryal-like protein-aided stabilization mechanism. The 3'-RCCA sequence of tRNA, which is a key recognition element for bacterial RNase P, is dispensable for tRNA recognition by MjaRNase P. The overall organization of MjaRNase P, particularly within the active site, is similar to those of bacterial and eukaryal RNase Ps, suggesting a universal catalytic mechanism for all RNase Ps.
| + | |
| - | | + | |
| - | Cryo-electron microscopy structure of an archaeal ribonuclease P holoenzyme.,Wan F, Wang Q, Tan J, Tan M, Chen J, Shi S, Lan P, Wu J, Lei M Nat Commun. 2019 Jun 13;10(1):2617. doi: 10.1038/s41467-019-10496-3. PMID:31197137<ref>PMID:31197137</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6k0b" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| | *[[Ribonuclease 3D structures|Ribonuclease 3D structures]] | | *[[Ribonuclease 3D structures|Ribonuclease 3D structures]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| | + | [[Category: Escherichia coli]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Metja]] | + | [[Category: Methanocaldococcus jannaschii]] |
| - | [[Category: Ribonuclease P]] | + | [[Category: Methanocaldococcus jannaschii DSM 2661]] |
| - | [[Category: Lan, P]] | + | [[Category: Lan P]] |
| - | [[Category: Lei, M]] | + | [[Category: Lei M]] |
| - | [[Category: Wan, F]] | + | [[Category: Wan F]] |
| - | [[Category: Wu, J]] | + | [[Category: Wu J]] |
| - | [[Category: Ribonuclease p]]
| + | |
| - | [[Category: Rna binding protein-rna complex]]
| + | |
| - | [[Category: Rna-protein complex]]
| + | |
