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| | <StructureSection load='6k2z' size='340' side='right'caption='[[6k2z]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='6k2z' size='340' side='right'caption='[[6k2z]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6k2z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K2Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6k2z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K2Z FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LGALS14, PPL13 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene>, <scene name='pdbligand=PRD_900008:alpha-lactose'>PRD_900008</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k2z OCA], [https://pdbe.org/6k2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k2z RCSB], [https://www.ebi.ac.uk/pdbsum/6k2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k2z ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k2z OCA], [https://pdbe.org/6k2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k2z RCSB], [https://www.ebi.ac.uk/pdbsum/6k2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k2z ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/PPL13_HUMAN PPL13_HUMAN]] Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis.<ref>PMID:19497882</ref>
| + | [https://www.uniprot.org/uniprot/PPL13_HUMAN PPL13_HUMAN] Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis.<ref>PMID:19497882</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The expression of prototype galectin-14 (Gal-14) in human placenta is higher than any other galectin, suggesting that it may play a role in fetal development and regulation of immune tolerance during pregnancy. Here, we solved the crystal structure of dimeric Gal-14, and found that its global fold is significantly different from that of other galectins with two beta-strands (S5 and S6) extending from one monomer and contributing to the carbohydrate binding domain of the other. The hemagglutination assay showed that this lectin could induce agglutination of chicken erythrocytes, even though lactose could not inhibit Gal-14-induced agglutination activity. Calorimetry indicates that lactose does not interact with this lectin. Compared to galectin-1, -3 and -8, Gal-14 has two key amino acids (a histidine and an arginine) in the normally conserved, canonical sugar binding site are substituted by glutamine (Gln53) and histidine (His57), thus likely explaining why lactose binding to this lectin is very weak. Lactose was observed in the ligand binding site of one Gal-14 structure, most likely because ligand binding is weak and crystals were allowed to grow over a long period of time in the presence of lactose. We also found that EGFP-tagged Gal-14 is primarily localized within the nucleus of different cell types. In addition, Gal-14 co-localized with c-Rel (a member of NF-kappaB family) in HeLa cells. These findings indicate that Gal-14 might regulate signal transduction pathways through NF-kappaB hubs. Overall, the present study provides impetus for further research into the function of Gal-14 in embryology.
| + | |
| - | | + | |
| - | Structure-function studies of galectin-14, an important effector molecule in embryology.,Si Y, Li Y, Yang T, Li X, Ayala GJ, Mayo KH, Tai G, Su J, Zhou Y FEBS J. 2020 Jun 11. doi: 10.1111/febs.15441. PMID:32525264<ref>PMID:32525264</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6k2z" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Su, J]] | + | [[Category: Su J]] |
| - | [[Category: Human galectin-14 with lactose]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
6k2z is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
PPL13_HUMAN Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis.[1]
See Also
References
- ↑ Than NG, Romero R, Goodman M, Weckle A, Xing J, Dong Z, Xu Y, Tarquini F, Szilagyi A, Gal P, Hou Z, Tarca AL, Kim CJ, Kim JS, Haidarian S, Uddin M, Bohn H, Benirschke K, Santolaya-Forgas J, Grossman LI, Erez O, Hassan SS, Zavodszky P, Papp Z, Wildman DE. A primate subfamily of galectins expressed at the maternal-fetal interface that promote immune cell death. Proc Natl Acad Sci U S A. 2009 Jun 16;106(24):9731-6. doi:, 10.1073/pnas.0903568106. Epub 2009 Jun 2. PMID:19497882 doi:http://dx.doi.org/10.1073/pnas.0903568106
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