6lep

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Current revision (10:46, 27 March 2024) (edit) (undo)
 
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<StructureSection load='6lep' size='340' side='right'caption='[[6lep]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
<StructureSection load='6lep' size='340' side='right'caption='[[6lep]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6lep]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_700085 Atcc 700085]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LEP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LEP FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6lep]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Spirochaeta_thermophila Spirochaeta thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LEP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LEP FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=THJ:THIOSULFATE'>THJ</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Spith_0734 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=154 ATCC 700085])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=THJ:THIOSULFATE'>THJ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lep OCA], [http://pdbe.org/6lep PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lep RCSB], [http://www.ebi.ac.uk/pdbsum/6lep PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lep ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lep OCA], [https://pdbe.org/6lep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lep RCSB], [https://www.ebi.ac.uk/pdbsum/6lep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lep ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/G0GAP6_SPITZ G0GAP6_SPITZ]
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We have demonstrated that a bacterial membrane protein, YeeE, mediates thiosulfate uptake. Thiosulfate is used for cysteine synthesis in bacteria as an inorganic sulfur source in the global biological sulfur cycle. The crystal structure of YeeE at 2.5-A resolution reveals an unprecedented hourglass-like architecture with thiosulfate in the positively charged outer concave side. YeeE is composed of loops and 13 helices including 9 transmembrane alpha helices, most of which show an intramolecular pseudo 222 symmetry. Four characteristic loops are buried toward the center of YeeE and form its central region surrounded by the nine helices. Additional electron density maps and successive molecular dynamics simulations imply that thiosulfate can remain temporally at several positions in the proposed pathway. We propose a plausible mechanism of thiosulfate uptake via three important conserved cysteine residues of the loops along the pathway.
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Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake.,Tanaka Y, Yoshikaie K, Takeuchi A, Ichikawa M, Mori T, Uchino S, Sugano Y, Hakoshima T, Takagi H, Nonaka G, Tsukazaki T Sci Adv. 2020 Aug 26;6(35):eaba7637. doi: 10.1126/sciadv.aba7637. eCollection, 2020 Aug. PMID:32923628<ref>PMID:32923628</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6lep" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 700085]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Sugano, Y]]
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[[Category: Spirochaeta thermophila]]
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[[Category: Takeuchi, A]]
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[[Category: Sugano Y]]
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[[Category: Tanaka, Y]]
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[[Category: Takeuchi A]]
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[[Category: Tsukazaki, T]]
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[[Category: Tanaka Y]]
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[[Category: Uchino, S]]
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[[Category: Tsukazaki T]]
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[[Category: Yoshikaie, K]]
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[[Category: Uchino S]]
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[[Category: Transmembrane]]
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[[Category: Yoshikaie K]]
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[[Category: Transport protein]]
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[[Category: Transporter]]
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Current revision

Crystal structure of thiosulfate transporter YeeE inactive mutant - C91A

PDB ID 6lep

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