6lgp

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<StructureSection load='6lgp' size='340' side='right'caption='[[6lgp]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
<StructureSection load='6lgp' size='340' side='right'caption='[[6lgp]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6lgp]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LGP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LGP FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6lgp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LGP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LGP FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLC:DIUNDECYL+PHOSPHATIDYL+CHOLINE'>PLC</scene>, <scene name='pdbligand=POV:(2S)-3-(HEXADECANOYLOXY)-2-[(9Z)-OCTADEC-9-ENOYLOXY]PROPYL+2-(TRIMETHYLAMMONIO)ETHYL+PHOSPHATE'>POV</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Trpv3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLC:DIUNDECYL+PHOSPHATIDYL+CHOLINE'>PLC</scene>, <scene name='pdbligand=POV:(2S)-3-(HEXADECANOYLOXY)-2-[(9Z)-OCTADEC-9-ENOYLOXY]PROPYL+2-(TRIMETHYLAMMONIO)ETHYL+PHOSPHATE'>POV</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lgp OCA], [http://pdbe.org/6lgp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lgp RCSB], [http://www.ebi.ac.uk/pdbsum/6lgp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lgp ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lgp OCA], [https://pdbe.org/6lgp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lgp RCSB], [https://www.ebi.ac.uk/pdbsum/6lgp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lgp ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/TRPV3_MOUSE TRPV3_MOUSE]] Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. Is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression (catagen) (By similarity).
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[https://www.uniprot.org/uniprot/TRPV3_MOUSE TRPV3_MOUSE] Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. Is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression (catagen) (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Transient receptor potential vanilloid subfamily member 3 (TRPV3) is a temperature-sensitive cation channel. Previous cryo-EM analyses of TRPV3 in detergent micelles or amphipol proposed that the lower gate opens by alpha-to-pi helical transitions of the nearby S6 helix. However, it remains unclear how physiological lipids are involved in the TRPV3 activation. Here we determined the apo state structure of mouse (Mus musculus) TRPV3 in a lipid nanodisc at 3.3 A resolution. The structure revealed that lipids bound to the pore domain stabilize the selectivity filter in the narrow state, suggesting that the selectivity filter of TRPV3 affects cation permeation. When the lower gate is closed in nanodisc-reconstituted TRPV3, the S6 helix adopts the pi-helical conformation without agonist- or heat-sensitization, potentially stabilized by putative intra-subunit hydrogen bonds and lipid binding. Our findings provide insights into the lipid-associated gating mechanism of TRPV3.
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The structure of lipid nanodisc-reconstituted TRPV3 reveals the gating mechanism.,Shimada H, Kusakizako T, Dung Nguyen TH, Nishizawa T, Hino T, Tominaga M, Nureki O Nat Struct Mol Biol. 2020 Jun 22. pii: 10.1038/s41594-020-0439-z. doi:, 10.1038/s41594-020-0439-z. PMID:32572254<ref>PMID:32572254</ref>
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==See Also==
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6lgp" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Lk3 transgenic mice]]
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[[Category: Mus musculus]]
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[[Category: Kusakizako, T]]
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[[Category: Kusakizako T]]
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[[Category: Nishizawa, T]]
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[[Category: Nishizawa T]]
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[[Category: Nureki, O]]
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[[Category: Nureki O]]
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[[Category: Shimada, H]]
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[[Category: Shimada H]]
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[[Category: Membrane protein]]
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[[Category: Nanodisc]]
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[[Category: Trp channel]]
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[[Category: Trpv family]]
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[[Category: Trpv3]]
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Revision as of 10:46, 27 March 2024

cryo-EM structure of TRPV3 in lipid nanodisc

PDB ID 6lgp

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