6lv0

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Current revision (10:47, 27 March 2024) (edit) (undo)
 
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<StructureSection load='6lv0' size='340' side='right'caption='[[6lv0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='6lv0' size='340' side='right'caption='[[6lv0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6lv0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_tropici_ciat_899 Rhizobium tropici ciat 899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LV0 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6lv0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_tropici_CIAT_899 Rhizobium tropici CIAT 899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LV0 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lpiA, RTCIAT899_CH14740 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=698761 Rhizobium tropici CIAT 899])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.998&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lv0 OCA], [https://pdbe.org/6lv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lv0 RCSB], [https://www.ebi.ac.uk/pdbsum/6lv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lv0 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lv0 OCA], [https://pdbe.org/6lv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lv0 RCSB], [https://www.ebi.ac.uk/pdbsum/6lv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lv0 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A6P1C618_RHITR A0A6P1C618_RHITR]
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As a large family of membrane proteins crucial for bacterial physiology and virulence, the Multiple Peptide Resistance Factors (MprFs) utilize two separate domains to synthesize and translocate aminoacyl phospholipids to the outer leaflets of bacterial membranes. The function of MprFs enables Staphylococcus aureus and other pathogenic bacteria to acquire resistance to daptomycin and cationic antimicrobial peptides. Here we present cryo-electron microscopy structures of MprF homodimer from Rhizobium tropici (RtMprF) at two different states in complex with lysyl-phosphatidylglycerol (LysPG). RtMprF contains a membrane-embedded lipid-flippase domain with two deep cavities opening toward the inner and outer leaflets of the membrane respectively. Intriguingly, a hook-shaped LysPG molecule is trapped inside the inner cavity with its head group bent toward the outer cavity which hosts a second phospholipid-binding site. Moreover, RtMprF exhibits multiple conformational states with the synthase domain adopting distinct positions relative to the flippase domain. Our results provide a detailed framework for understanding the mechanisms of MprF-mediated modification and translocation of phospholipids.
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Phospholipid translocation captured in a bifunctional membrane protein MprF.,Song D, Jiao H, Liu Z Nat Commun. 2021 May 18;12(1):2927. doi: 10.1038/s41467-021-23248-z. PMID:34006869<ref>PMID:34006869</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6lv0" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Rhizobium tropici ciat 899]]
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[[Category: Rhizobium tropici CIAT 899]]
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[[Category: Jiao, H Z]]
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[[Category: Jiao HZ]]
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[[Category: Liu, Z F]]
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[[Category: Liu ZF]]
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[[Category: Song, D F]]
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[[Category: Song DF]]
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[[Category: Biosynthetic protein]]
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[[Category: Lipid biosynthesis]]
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Current revision

Crystal structure of the soluble domain of the multiple peptide resistance factor (MprF) from Rhizobium tropici

PDB ID 6lv0

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OCA

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