1fje

<StructureSection load='1fje' size='340' side='right'caption='[[1fje]], [[NMR_Ensembles_of_Models | 19 NMR models]]' scene=''>

<table><tr><td colspan='2'>[[1fje]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Golden_hamster Golden hamster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FJE FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1fj7|1fj7]], [[1fjc|1fjc]]</div></td></tr>

[[https://www.uniprot.org/uniprot/NUCL_MESAU NUCL_MESAU]] Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats (By similarity).<ref>PMID:3409881</ref>

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

The structure of the 28 kDa complex of the first two RNA binding domains (RBDs) of nucleolin (RBD12) with an RNA stem-loop that includes the nucleolin recognition element UCCCGA in the loop was determined by NMR spectroscopy. The structure of nucleolin RBD12 with the nucleolin recognition element (NRE) reveals that the two RBDs bind on opposite sides of the RNA loop, forming a molecular clamp that brings the 5' and 3' ends of the recognition sequence close together and stabilizing the stem-loop. The specific interactions observed in the structure explain the sequence specificity for the NRE sequence. Binding studies of mutant proteins and analysis of conserved residues support the proposed interactions. The mode of interaction of the protein with the RNA and the location of the putative NRE sites suggest that nucleolin may function as an RNA chaperone to prevent improper folding of the nascent pre-rRNA.

Molecular basis of sequence-specific recognition of pre-ribosomal RNA by nucleolin.,Allain FH, Bouvet P, Dieckmann T, Feigon J EMBO J. 2000 Dec 15;19(24):6870-81. PMID:11118222<ref>PMID:11118222</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

</div>

<div class="pdbe-citations 1fje" style="background-color:#fffaf0;"></div>

[[Category: Golden hamster]]

[[Category: Allain, F H.T]]

[[Category: Bouvet, P]]

[[Category: Dieckmann, T]]

[[Category: Feigon, J]]

[[Category: Structural protein-rna complex]]