1ttx

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Revision as of 17:21, 12 November 2007

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Solution Stucture of human beta parvalbumin (oncomodulin) refined with a paramagnetism based strategy

Overview

The aim of this research was to determine the structure of human, beta-parvalbumin (109 amino acids) and to compare it with its paralog and, ortholog proteins. The structure was determined in solution using, multinuclear and multidimensional NMR methods and refined using, substitution of the EF-hand Ca(2+) ion with a paramagnetic lanthanide. The, resulting family of structures had a backbone rmsd of 0.50 A. Comparison, with rat oncomodulin (X-ray, 1.3 A resolution) as well as with human (NMR, backbone rmsd of 0.49 A) and rat (X-ray, 2.0 A resolution) parvalbumins, reveals small but reliable local differences, often but not always related, to amino acid variability. The analysis of these structures has led us to, propose an explanation for the different affinity for Ca(2+) between, alpha- and beta-parvalbumins and between parvalbumins and calmodulins.

About this Structure

1TTX is a Single protein structure of sequence from Homo sapiens with CA as ligand. Full crystallographic information is available from OCA.

Reference

Solution structure of human beta-parvalbumin and structural comparison with its paralog alpha-parvalbumin and with their rat orthologs., Babini E, Bertini I, Capozzi F, Del Bianco C, Hollender D, Kiss T, Luchinat C, Quattrone A, Biochemistry. 2004 Dec 28;43(51):16076-85. PMID:15610002

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