This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1tv9
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /> <applet load="1tv9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tv9, resolution 2.00Å" /> '''HUMAN DNA POLYMERAS...)
Next diff →
Revision as of 17:21, 12 November 2007
|
HUMAN DNA POLYMERASE BETA COMPLEXED WITH NICKED DNA CONTAINING A MISMATCHED TEMPLATE ADENINE AND INCOMING CYTIDINE
Overview
DNA polymerases generally select the correct nucleotide from a pool of, structurally similar molecules to preserve Watson-Crick base-pairing, rules. We report the structure of DNA polymerase beta with DNA mismatches, situated in the polymerase active site. This was achieved by using nicked, product DNA that traps the mispair (template-primer, A-C or T-C) in the, nascent base pair binding pocket. The structure of each mispair complex, indicates that the bases do not form hydrogen bonds with one another, but, form a staggered arrangement where the bases of the mispair partially, overlap. This prevents closure/opening of the N subdomain that is believed, to be required for catalytic cycling. The partially open conformation of, the N subdomain results in distinct hydrogen bonding networks that are, unique for each mispair. These structures define diverse molecular aspects, of misinsertion that are consistent with the induced-fit model for, substrate specificity.
About this Structure
1TV9 is a Single protein structure of sequence from Homo sapiens with MG, NA and PO4 as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
Reference
Structural insights into DNA polymerase beta deterrents for misincorporation support an induced-fit mechanism for fidelity., Krahn JM, Beard WA, Wilson SH, Structure. 2004 Oct;12(10):1823-32. PMID:15458631
Page seeded by OCA on Mon Nov 12 19:28:22 2007
