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| - | ==NMR solution structure of the 2:1 complex of a platinum(II) compound bound to Myc1234 G-quadruplex reveals the mechanism of conformational switch and dynamic binding of G-quadruplex==
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| - | <StructureSection load='7djv' size='340' side='right'caption='[[7djv]]' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[7djv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DJV FirstGlance]. <br>
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| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=H9C:Bis(1,10-phenanthroline)platinum(II)'>H9C</scene></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7djv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7djv OCA], [https://pdbe.org/7djv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7djv RCSB], [https://www.ebi.ac.uk/pdbsum/7djv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7djv ProSAT]</span></td></tr>
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| - | </table>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | G-quadruplex (G4) transitions play integral roles in regulating biological functions and can be modified by ligands. However, little is known about G4 transitions. Herein, we reveal distinct pathways of a platinum(II) compound Pt-phen converting parallel-stranded MYC G4 to a hybrid-type structure. Three NMR structures, 1:1 5'-end binding, 1:1 3'-end binding and 2:1 Pt-phen-MYC G4 complexes, were determined by NMR. We find that Pt-phen drives G4 transition at a low ratio. Under physiological 100 mM K+ conditions, a significant stable hydrogen-bonded T:T:A triad is formed at 3'-end of hybrid-type Myc1234, and consequently, Pt-phen first binds the 5'-end to form a 1:1 5'-end binding complex and then disrupts the 3' T:T:A triad and binds 3'-end to form a 2:1 complex with more Pt-phen. Remarkably, the G4 transition pathway is different in 5 mM K+ with Pt-phen first binding the 3'-end and then the 5'-end. 'Edgewise-loop and flanking/ligand/G-tetrad' sandwich structure formation and terminal T:T:A triad stabilization play decisive roles in advancing and altering transition pathways. Our work is the first to elucidate the molecular structures of G4 transitions driven by a small molecule. The ligand-driven G4 transition is a dynamic process that includes a quick G4 transition and multiple complexes formation.
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| - | G-quadruplex structural transition driven by a platinum compound.,Liu W, Zhu BC, Liu LY, Xia XY, Mao ZW Nucleic Acids Res. 2022 Aug 12;50(14):7816-7828. doi: 10.1093/nar/gkac572. PMID:35766415<ref>PMID:35766415</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 7djv" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Homo sapiens]]
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| - | [[Category: Large Structures]]
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| - | [[Category: Liu W]]
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| - | [[Category: Mao ZW]]
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