1rfo
From Proteopedia
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'''Trimeric Foldon of the T4 phagehead fibritin''' | '''Trimeric Foldon of the T4 phagehead fibritin''' | ||
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[[Category: Meier, S.]] | [[Category: Meier, S.]] | ||
[[Category: Moglich, A.]] | [[Category: Moglich, A.]] | ||
| - | [[Category: | + | [[Category: Beta hairpin]] |
| - | [[Category: | + | [[Category: Trimer]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:26:27 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 04:26, 3 May 2008
Trimeric Foldon of the T4 phagehead fibritin
Overview
The foldon domain constitutes the C-terminal 30 amino acid residues of the trimeric protein fibritin from bacteriophage T4. Its function is to promote folding and trimerization of fibritin. We investigated structure, stability and folding mechanism of the isolated foldon domain. The domain folds into the same trimeric beta-propeller structure as in fibritin and undergoes a two-state unfolding transition from folded trimer to unfolded monomers. The folding kinetics involve several consecutive reactions. Structure formation in the region of the single beta-hairpin of each monomer occurs on the submillisecond timescale. This reaction is followed by two consecutive association steps with rate constants of 1.9(+/-0.5)x10(6)M(-1)s(-1) and 5.4(+/-0.3)x10(6)M(-1)s(-1) at 0.58 M GdmCl, respectively. This is similar to the fastest reported bimolecular association reactions for folding of dimeric proteins. At low concentrations of protein, folding shows apparent third-order kinetics. At high concentrations of protein, the reaction becomes almost independent of protein concentrations with a half-time of about 3 ms, indicating that a first-order folding step from a partially folded trimer to the native protein (k=210 +/- 20 s(-1)) becomes rate-limiting. Our results suggest that all steps on the folding/trimerization pathway of the foldon domain are evolutionarily optimized for rapid and specific initiation of trimer formation during fibritin assembly. The results further show that beta-hairpins allow efficient and rapid protein-protein interactions during folding.
About this Structure
1RFO is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
Reference
Very fast folding and association of a trimerization domain from bacteriophage T4 fibritin., Guthe S, Kapinos L, Moglich A, Meier S, Grzesiek S, Kiefhaber T, J Mol Biol. 2004 Apr 2;337(4):905-15. PMID:15033360 Page seeded by OCA on Sat May 3 07:26:27 2008
