|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal Structure of human ST6GALNAC2 in complex with CMP== | | ==Crystal Structure of human ST6GALNAC2 in complex with CMP== |
| - | <StructureSection load='6apl' size='340' side='right' caption='[[6apl]], [[Resolution|resolution]] 2.35Å' scene=''> | + | <StructureSection load='6apl' size='340' side='right'caption='[[6apl]], [[Resolution|resolution]] 2.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6apl]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6APL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6apl]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6APL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ST6GALNAC2, SIAT7B, SIATL1, STHM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6apl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6apl OCA], [https://pdbe.org/6apl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6apl RCSB], [https://www.ebi.ac.uk/pdbsum/6apl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6apl ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6apl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6apl OCA], [http://pdbe.org/6apl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6apl RCSB], [http://www.ebi.ac.uk/pdbsum/6apl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6apl ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/SIA7B_HUMAN SIA7B_HUMAN] |
| - | Vertebrate glycoproteins and glycolipids are synthesized in complex biosynthetic pathways localized predominantly within membrane compartments of the secretory pathway. The enzymes that catalyze these reactions are exquisitely specific, yet few have been extensively characterized because of challenges associated with their recombinant expression as functional products. We used a modular approach to create an expression vector library encoding all known human glycosyltransferases, glycoside hydrolases, and sulfotransferases, as well as other glycan-modifying enzymes. We then expressed the enzymes as secreted catalytic domain fusion proteins in mammalian and insect cell hosts, purified and characterized a subset of the enzymes, and determined the structure of one enzyme, the sialyltransferase ST6GalNAcII. Many enzymes were produced at high yields and at similar levels in both hosts, but individual protein expression levels varied widely. This expression vector library will be a transformative resource for recombinant enzyme production, broadly enabling structure-function studies and expanding applications of these enzymes in glycochemistry and glycobiology.
| + | |
| | | | |
| - | Expression system for structural and functional studies of human glycosylation enzymes.,Moremen KW, Ramiah A, Stuart M, Steel J, Meng L, Forouhar F, Moniz HA, Gahlay G, Gao Z, Chapla D, Wang S, Yang JY, Prabhakar PK, Johnson R, Rosa MD, Geisler C, Nairn AV, Seetharaman J, Wu SC, Tong L, Gilbert HJ, LaBaer J, Jarvis DL Nat Chem Biol. 2017 Dec 18. pii: nchembio.2539. doi: 10.1038/nchembio.2539. PMID:29251719<ref>PMID:29251719</ref>
| + | ==See Also== |
| - | | + | *[[Sialyltransferase 3D structures|Sialyltransferase 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6apl" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Forouhar, F]] | + | [[Category: Large Structures]] |
| - | [[Category: Moremen, K W]] | + | [[Category: Forouhar F]] |
| - | [[Category: Structural genomic]] | + | [[Category: Moremen KW]] |
| - | [[Category: Tong, L]] | + | [[Category: Tong L]] |
| - | [[Category: Glycosyltransferase]]
| + | |
| - | [[Category: Nesg]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Transferase]]
| + | |
