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| | <StructureSection load='6oil' size='340' side='right'caption='[[6oil]], [[Resolution|resolution]] 1.85Å' scene=''> | | <StructureSection load='6oil' size='340' side='right'caption='[[6oil]], [[Resolution|resolution]] 1.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6oil]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OIL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OIL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6oil]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OIL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VSIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6oil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oil OCA], [http://pdbe.org/6oil PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oil RCSB], [http://www.ebi.ac.uk/pdbsum/6oil PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oil ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oil OCA], [https://pdbe.org/6oil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oil RCSB], [https://www.ebi.ac.uk/pdbsum/6oil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oil ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/VISTA_HUMAN VISTA_HUMAN]] Immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). May stimulate MMP14-mediated MMP2 activation (PubMed:20666777).[UniProtKB:Q9D659]<ref>PMID:20666777</ref> <ref>PMID:24691993</ref> | + | [https://www.uniprot.org/uniprot/VISTA_HUMAN VISTA_HUMAN] Immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). May stimulate MMP14-mediated MMP2 activation (PubMed:20666777).[UniProtKB:Q9D659]<ref>PMID:20666777</ref> <ref>PMID:24691993</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | V-domain immunoglobulin (Ig) suppressor of T cell activation (VISTA) is an immune checkpoint protein that inhibits the T cell response against cancer. Similar to PD-1 and CTLA-4, a blockade of VISTA promotes tumor clearance by the immune system. Here, we report a 1.85 A crystal structure of the elusive human VISTA extracellular domain, whose lack of homology necessitated a combinatorial MR-Rosetta approach for structure determination. We highlight features that make the VISTA immunoglobulin variable (IgV)-like fold unique among B7 family members, including two additional disulfide bonds and an extended loop region with an attached helix that we show forms a contiguous binding epitope for a clinically relevant anti-VISTA antibody. We propose an overlap of this antibody-binding region with the binding epitope for V-set and Ig domain containing 3 (VSIG3), a purported functional binding partner of VISTA. The structure and functional epitope presented here will help guide future drug development efforts against this important checkpoint target.
| + | |
| - | | + | |
| - | Structure and Functional Binding Epitope of V-domain Ig Suppressor of T Cell Activation.,Mehta N, Maddineni S, Mathews II, Andres Parra Sperberg R, Huang PS, Cochran JR Cell Rep. 2019 Sep 3;28(10):2509-2516.e5. doi: 10.1016/j.celrep.2019.07.073. PMID:31484064<ref>PMID:31484064</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6oil" style="background-color:#fffaf0;"></div>
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cochran, J R]] | + | [[Category: Cochran JR]] |
| - | [[Category: Mathews, I I]] | + | [[Category: Mathews II]] |
| - | [[Category: Mehta, N]] | + | [[Category: Mehta N]] |
| - | [[Category: Signaling protein]]
| + | |
| - | [[Category: Suppressor]]
| + | |
| - | [[Category: T cell activation]]
| + | |
| - | [[Category: Transmembrane]]
| + | |
